|
rdf:type |
|
|
lifeskim:mentions |
|
|
pubmed:issue |
1
|
|
pubmed:dateCreated |
2001-1-17
|
|
pubmed:abstractText |
Cl(-) currents induced by cell swelling were characterized in an immortalized cell line (DC1) derived from rabbit distal bright convoluted tubule by the whole cell patch-clamp techniques and by (125)I(-) efflux experiments. Exposure of cells to a hypotonic shock induced outwardly rectifying Cl(-) currents that could be blocked by 0.1 mM 5-nitro-2-(3-phenylpropyl-amino)benzoic acid, 1 mM DIDS, and by 1 mM diphenylamine-2-carboxylate. (125)I(-) efflux experiments showed that exposure of the monolayer to a hypotonic medium increased (125)I(-) loss. Preincubation of cells with LaCl(3) or GdCl(3) prevented the development of the response. The addition of 10 microM adenosine to the bath medium activated outwardly rectifying whole cell currents similar to those recorded after hypotonic shock. This conductance was inhibited by the A(1)-receptor antagonist 8-cyclopentyl-1,3-diproxylxanthine (DPCPX), LaCl(3), or GdCl(3) and was activated by GTPgammaS. The selective A(1)-receptor agonist N(6)-cyclopentyladenosine (CPA) mimicked the effect of hypotonicity on (125)I(-) efflux. The CPA-induced increase of (125)I(-) efflux was inhibited by DPCPX and external application of LaCl(3) or GdCl(3). Adenosine also enhanced Mn(2+) influx across the apical membrane. Overall, the data show that DC1 cells possess swelling- and adenosine-activated Cl(-) conductances that share identical characteristics. The activation of both conductances involved Ca(2+) entry into the cell, probably via mechanosensitive Ca(2+) channels. The effects of adenosine are mediated via A(1) receptors that could mediate the purinergic regulation of the volume-sensitive Cl(-) conductance.
|
|
pubmed:language |
eng
|
|
pubmed:journal |
|
|
pubmed:citationSubset |
IM
|
|
pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/1,3-dipropyl-8-cyclopentylxanthine,
http://linkedlifedata.com/resource/pubmed/chemical/Adenosine,
http://linkedlifedata.com/resource/pubmed/chemical/Chloride Channels,
http://linkedlifedata.com/resource/pubmed/chemical/Gadolinium,
http://linkedlifedata.com/resource/pubmed/chemical/Guanosine 5'-O-(3-Thiotriphosphate),
http://linkedlifedata.com/resource/pubmed/chemical/Hypotonic Solutions,
http://linkedlifedata.com/resource/pubmed/chemical/Iodine Radioisotopes,
http://linkedlifedata.com/resource/pubmed/chemical/Lanthanum,
http://linkedlifedata.com/resource/pubmed/chemical/Manganese,
http://linkedlifedata.com/resource/pubmed/chemical/N(6)-cyclopentyladenosine,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Purinergic P1,
http://linkedlifedata.com/resource/pubmed/chemical/Recombinant Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Xanthines,
http://linkedlifedata.com/resource/pubmed/chemical/gadolinium chloride,
http://linkedlifedata.com/resource/pubmed/chemical/lanthanum chloride
|
|
pubmed:status |
MEDLINE
|
|
pubmed:month |
Jan
|
|
pubmed:issn |
1931-857X
|
|
pubmed:author |
|
|
pubmed:issnType |
Print
|
|
pubmed:volume |
280
|
|
pubmed:owner |
NLM
|
|
pubmed:authorsComplete |
Y
|
|
pubmed:pagination |
F126-45
|
|
pubmed:dateRevised |
2011-4-28
|
|
pubmed:meshHeading |
pubmed-meshheading:11133523-Adenosine,
pubmed-meshheading:11133523-Animals,
pubmed-meshheading:11133523-Cell Line,
pubmed-meshheading:11133523-Cell Membrane,
pubmed-meshheading:11133523-Cell Size,
pubmed-meshheading:11133523-Chloride Channels,
pubmed-meshheading:11133523-Gadolinium,
pubmed-meshheading:11133523-Guanosine 5'-O-(3-Thiotriphosphate),
pubmed-meshheading:11133523-Hypotonic Solutions,
pubmed-meshheading:11133523-Iodine Radioisotopes,
pubmed-meshheading:11133523-Kidney Tubules,
pubmed-meshheading:11133523-Lanthanum,
pubmed-meshheading:11133523-Manganese,
pubmed-meshheading:11133523-Membrane Potentials,
pubmed-meshheading:11133523-Patch-Clamp Techniques,
pubmed-meshheading:11133523-Rabbits,
pubmed-meshheading:11133523-Receptors, Purinergic P1,
pubmed-meshheading:11133523-Recombinant Proteins,
pubmed-meshheading:11133523-Transfection,
pubmed-meshheading:11133523-Xanthines
|
|
pubmed:year |
2001
|
|
pubmed:articleTitle |
Extracellular adenosine modulates a volume-sensitive-like chloride conductance in immortalized rabbit DC1 cells.
|
|
pubmed:affiliation |
Unité Mixte de Recherche Centre National de la Recherche Scientifique 6548, Université de Nice-Sophia Antipolis, O6108 Nice Cedex 2, France.
|
|
pubmed:publicationType |
Journal Article
|
