Source:http://linkedlifedata.com/resource/pubmed/id/11132930
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
3
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pubmed:dateCreated |
2000-12-29
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pubmed:abstractText |
The expression of thrombospondin-1 (TSP-1) and its role in gliomas have not been well examined. In the present study TSP-1 expression in a panel of malignant glioma cell lines and the expression of TSP-1 and transforming growth factor (TGF-beta) proteins in low-grade and malignant glioma tissues were investigated. Reverse transcription-polymerase chain reaction analysis showed that nine of nine malignant glioma cell lines expressed TSP-1 mRNA, and seven of nine glioma lines expressed TSP-2 mRNA. Production and secretion of TSP-1 were examined in the T98G glioblastoma cell line by western blot analysis. Total TSP-1 protein content in the supernatant was 10 times higher than that in the cell lysate. Secretion of TSP-1 was examined in these glioma cell lines by western blot analysis. All glioma lines secreted significant levels of TSP-1. Bioassay showed that all tumor lines had the capacity to activate latent TGF-beta. Localization of TSP-1, TGF-beta1, -beta2, and -beta3 was examined immunohistochemically in surgically resected glioma tissues, including 11 glioblastomas, six anaplastic astrocytomas, and eight astrocytomas. Most glioblastomas expressed high levels of both TSP-1 and TGF-beta. Anaplastic astrocytomas expressed moderate levels of TSP-1 and TGF-beta. Most malignant gliomas expressed various levels of TGF-beta1, -beta2, and -beta3. The expression of both proteins, however, was weak in low-grade gliomas. Normal brain tissues around the tumors were negatively or very weakly positively stained for TSP-1 and TGF-beta. These results indicate that most malignant glioma cells express TSP-1 in vitro and in vivo, and the expression of TSP-1 and TGF-beta in vivo correlates with the histologic malignancy of glioma. Overexpression of both TSP-1 and TGF-beta may increase the biologic malignancy of malignant gliomas, through generating the active form of TGF-beta in tumor tissues.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger,
http://linkedlifedata.com/resource/pubmed/chemical/Thrombospondin 1,
http://linkedlifedata.com/resource/pubmed/chemical/Thrombospondins,
http://linkedlifedata.com/resource/pubmed/chemical/Transforming Growth Factor beta,
http://linkedlifedata.com/resource/pubmed/chemical/Tumor Markers, Biological,
http://linkedlifedata.com/resource/pubmed/chemical/thrombospondin 2
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pubmed:status |
MEDLINE
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pubmed:month |
Sep
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pubmed:issn |
0919-6544
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
20
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
161-9
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pubmed:dateRevised |
2008-3-10
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pubmed:meshHeading |
pubmed-meshheading:11132930-Astrocytoma,
pubmed-meshheading:11132930-Diagnosis, Differential,
pubmed-meshheading:11132930-Glioblastoma,
pubmed-meshheading:11132930-Glioma,
pubmed-meshheading:11132930-Humans,
pubmed-meshheading:11132930-RNA, Messenger,
pubmed-meshheading:11132930-Thrombospondin 1,
pubmed-meshheading:11132930-Thrombospondins,
pubmed-meshheading:11132930-Transforming Growth Factor beta,
pubmed-meshheading:11132930-Tumor Cells, Cultured,
pubmed-meshheading:11132930-Tumor Markers, Biological
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pubmed:year |
2000
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pubmed:articleTitle |
Correlation of thrombospondin-1 and transforming growth factor-beta expression with malignancy of glioma.
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pubmed:affiliation |
Department of Neurosurgery, Yamanashi Medical University, Japan.
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pubmed:publicationType |
Journal Article
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