Linked Life Data

11131389

Source:http://linkedlifedata.com/resource/pubmed/id/11131389

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
5
pubmed:dateCreated
2000-12-22
pubmed:abstractText
Escherichia coli strain HS3, metabolically engineered to have Met(-), AHV(r), Ile(L) and AEC(r) characteristics, produced 58.0 g/l of L-threonine, but it was neither salt-tolerant nor osmotolerant; and the growth and threonine production of the strain were severely inhibited both by the addition of NaCl with a concentration higher than 2% and by the presence of glucose with a concentration higher than 10%. Therefore, salt-tolerant mutants were isolated. The salt-tolerant mutants, HS454 and HS528 which were derived from strain HS3, were both tolerant to salt (2%) and hyper-productive. The growth and L-threonine production by the mutant strain HS454 were almost unaffected by a glucose concentration lower than 10%, but gradually reduced with increasing glucose concentration, up to 15%. However, the mutant strain HS528 showed slightly enhanced growth and L-threonine production with increasing glucose concentration, up to 10-12.5%. Strains HS454 and HS528 produced 69.8 g/l and 74.0 g/l of L-threonine, respectively in a 5-1 jar fermentor.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Nov
pubmed:issn
0175-7598
pubmed:author
pubmed:issnType
Print
pubmed:volume
54
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
647-51
pubmed:meshHeading
pubmed:year
2000
pubmed:articleTitle
Characterization of salt-tolerant mutant for enhancement of L-threonine production in Escherichia coli.
pubmed:affiliation
Department of Bioscience and Biotechnology, Hankuk University of Foreign Studies, Youngin, Kyungki-Do, Korea.
pubmed:publicationType
Journal Article