Linked Life Data

11131152

Source:http://linkedlifedata.com/resource/pubmed/id/11131152

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
4
pubmed:dateCreated
2000-12-22
pubmed:abstractText
The CD8 glycoprotein serves as a coreceptor for T cell receptor (TCR)-mediated recognition of peptides associated to MHC class I. In CD8alpha deficiency, MHC class-I restricted cytotoxic T lymphocytes (CTL) are absent and lineage commitment to CD8 cells is impaired. We described a pharmacological approach to reduce the amount of CD8 surface glycoproteins on human peripheral blood T cells. Using calyculin A, a potent inhibitor of protein phosphatases, we were able to down regulate both CD8alpha protein and mRNA. Reduction of CD8alpha surface expression was dose- and time-dependent. The effect was specific to CD8alpha in that CD4 expression was not affected and specific for calyculin A. Okadaic acid, another phosphatase inhibitor did not show any influence on the expression of CD4 or CD8.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Nov
pubmed:issn
0171-2985
pubmed:author
pubmed:issnType
Print
pubmed:volume
202
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
353-62
pubmed:dateRevised
2007-11-15
pubmed:meshHeading
pubmed:year
2000
pubmed:articleTitle
Calyculin A inhibits expression of CD8alpha but not CD4 in human peripheral blood T cells.
pubmed:affiliation
University of Konstanz, Faculty of Sciences, Department of Biology, Immunology, Germany.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't