| pubmed-article:11129658 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:11129658 | lifeskim:mentions | umls-concept:C0021311 | lld:lifeskim |
| pubmed-article:11129658 | lifeskim:mentions | umls-concept:C0035820 | lld:lifeskim |
| pubmed-article:11129658 | lifeskim:mentions | umls-concept:C0026912 | lld:lifeskim |
| pubmed-article:11129658 | lifeskim:mentions | umls-concept:C0028128 | lld:lifeskim |
| pubmed-article:11129658 | lifeskim:mentions | umls-concept:C0123759 | lld:lifeskim |
| pubmed-article:11129658 | lifeskim:mentions | umls-concept:C0024432 | lld:lifeskim |
| pubmed-article:11129658 | lifeskim:mentions | umls-concept:C0178719 | lld:lifeskim |
| pubmed-article:11129658 | lifeskim:mentions | umls-concept:C1456820 | lld:lifeskim |
| pubmed-article:11129658 | lifeskim:mentions | umls-concept:C0030685 | lld:lifeskim |
| pubmed-article:11129658 | lifeskim:mentions | umls-concept:C0024426 | lld:lifeskim |
| pubmed-article:11129658 | lifeskim:mentions | umls-concept:C0680255 | lld:lifeskim |
| pubmed-article:11129658 | lifeskim:mentions | umls-concept:C0205263 | lld:lifeskim |
| pubmed-article:11129658 | lifeskim:mentions | umls-concept:C0391871 | lld:lifeskim |
| pubmed-article:11129658 | lifeskim:mentions | umls-concept:C1283071 | lld:lifeskim |
| pubmed-article:11129658 | lifeskim:mentions | umls-concept:C1963578 | lld:lifeskim |
| pubmed-article:11129658 | pubmed:issue | 6 | lld:pubmed |
| pubmed-article:11129658 | pubmed:dateCreated | 2000-12-20 | lld:pubmed |
| pubmed-article:11129658 | pubmed:abstractText | IL-12 is believed to play an important role in cell-mediated immunity against intracellular infection primarily by acting on T and NK cells. Recent evidence has suggested, however, that IL-12 has broader cellular targets than previously thought. In this study, we examined the role of IL-12 in macrophage TNF-alpha and nitric oxide (NO) release by using an in vitro model of intracellular infection. IL-12 alone released relatively little TNF-alpha and NO, whereas live mycobacteria alone released TNF-alpha markedly but little NO from murine alveolar macrophages. However, IL-12 and mycobacteria together enhanced TNF-alpha and NO release synergistically. Because IL-12 and mycobacteria also released IFN-gamma from macrophages synergistically, and exogenous IFN-gamma with mycobacteria enhanced TNF-alpha and NO release synergistically, we examined the role of endogenous IFN-gamma in IL-12/mycobacteria-stimulated macrophage activation. Using macrophages from mice deficient in IFN-gamma, we found that IL-12/mycobacteria-enhanced macrophage TNF-alpha and NO release was mediated through endogenous IFN-gamma. We further demonstrated that IFN-gamma and mycobacteria together had a selective effect on macrophage cytokine release because they released TNF-alpha synergistically but not macrophage chemotactic protein-1 (MCP-1). These findings reveal that IL-12 can activate macrophages potently during intracellular infection, and this activating effect is mediated primarily through its effect on macrophage IFN-gamma release. | lld:pubmed |
| pubmed-article:11129658 | pubmed:language | eng | lld:pubmed |
| pubmed-article:11129658 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11129658 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:11129658 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11129658 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11129658 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11129658 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11129658 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11129658 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:11129658 | pubmed:month | Dec | lld:pubmed |
| pubmed-article:11129658 | pubmed:issn | 0741-5400 | lld:pubmed |
| pubmed-article:11129658 | pubmed:author | pubmed-author:VegaE TET | lld:pubmed |
| pubmed-article:11129658 | pubmed:author | pubmed-author:ZganiaczAA | lld:pubmed |
| pubmed-article:11129658 | pubmed:author | pubmed-author:SantosuossoMM | lld:pubmed |
| pubmed-article:11129658 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:11129658 | pubmed:volume | 68 | lld:pubmed |
| pubmed-article:11129658 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:11129658 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:11129658 | pubmed:pagination | 897-902 | lld:pubmed |
| pubmed-article:11129658 | pubmed:dateRevised | 2011-11-17 | lld:pubmed |
| pubmed-article:11129658 | pubmed:meshHeading | pubmed-meshheading:11129658... | lld:pubmed |
| pubmed-article:11129658 | pubmed:meshHeading | pubmed-meshheading:11129658... | lld:pubmed |
| pubmed-article:11129658 | pubmed:meshHeading | pubmed-meshheading:11129658... | lld:pubmed |
| pubmed-article:11129658 | pubmed:meshHeading | pubmed-meshheading:11129658... | lld:pubmed |
| pubmed-article:11129658 | pubmed:meshHeading | pubmed-meshheading:11129658... | lld:pubmed |
| pubmed-article:11129658 | pubmed:meshHeading | pubmed-meshheading:11129658... | lld:pubmed |
| pubmed-article:11129658 | pubmed:meshHeading | pubmed-meshheading:11129658... | lld:pubmed |
| pubmed-article:11129658 | pubmed:meshHeading | pubmed-meshheading:11129658... | lld:pubmed |
| pubmed-article:11129658 | pubmed:meshHeading | pubmed-meshheading:11129658... | lld:pubmed |
| pubmed-article:11129658 | pubmed:meshHeading | pubmed-meshheading:11129658... | lld:pubmed |
| pubmed-article:11129658 | pubmed:meshHeading | pubmed-meshheading:11129658... | lld:pubmed |
| pubmed-article:11129658 | pubmed:meshHeading | pubmed-meshheading:11129658... | lld:pubmed |
| pubmed-article:11129658 | pubmed:meshHeading | pubmed-meshheading:11129658... | lld:pubmed |
| pubmed-article:11129658 | pubmed:meshHeading | pubmed-meshheading:11129658... | lld:pubmed |
| pubmed-article:11129658 | pubmed:meshHeading | pubmed-meshheading:11129658... | lld:pubmed |
| pubmed-article:11129658 | pubmed:year | 2000 | lld:pubmed |
| pubmed-article:11129658 | pubmed:articleTitle | Role of IL-12 in macrophage activation during intracellular infection: IL-12 and mycobacteria synergistically release TNF-alpha and nitric oxide from macrophages via IFN-gamma induction. | lld:pubmed |
| pubmed-article:11129658 | pubmed:affiliation | Department of Pathology and Molecular Medicine, Centre for Gene Therapeutics, McMaster University, Hamilton, Ontario, Canada. xingz@fhs.mcmaster.ca | lld:pubmed |
| pubmed-article:11129658 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:11129658 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:11129658 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:11129658 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:11129658 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:11129658 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:11129658 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:11129658 | lld:pubmed |
