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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
6
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pubmed:dateCreated |
2000-12-20
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pubmed:abstractText |
This study was initiated to identify and characterize thyroid fibrosis in a murine model of granulomatous experimental autoimmune thyroiditis (G-EAT) and determine if TGF-beta1 might be involved in fibrosis. G-EAT was induced by transfer of mouse thyroglobulin-sensitized spleen cells activated in vitro with thyroglobulin, anti-IL-2R, and IL-12. There was almost complete destruction of thyroid follicles, leading to fibrosis of the gland and reduced serum T4 levels. Fibrosis was confirmed by staining for collagen and alpha smooth-muscle actin, a marker of myofibroblasts. Kinetic studies characterized the onset and development of thyroid fibrosis. TGF-1beta was increased at mRNA and protein levels, and expression of TGF-beta1 protein paralleled G-EAT severity. Comparison of staining patterns showed that TGF-beta1 was expressed in areas of myofibroblast and collagen accumulation, implying that TGF-beta1 may play a role in fibrosis in G-EAT. Further studies demonstrated that myofibroblasts, macrophages, and thyrocytes contributed to TGF-beta1 production. This provides an excellent model to study the mechanisms of fibrosis associated with autoimmune damage.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Actins,
http://linkedlifedata.com/resource/pubmed/chemical/Antibodies, Monoclonal,
http://linkedlifedata.com/resource/pubmed/chemical/Biological Markers,
http://linkedlifedata.com/resource/pubmed/chemical/Collagen,
http://linkedlifedata.com/resource/pubmed/chemical/Interleukin-12,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Interleukin-2,
http://linkedlifedata.com/resource/pubmed/chemical/Tgfb1 protein, mouse,
http://linkedlifedata.com/resource/pubmed/chemical/Thyroglobulin,
http://linkedlifedata.com/resource/pubmed/chemical/Thyroxine,
http://linkedlifedata.com/resource/pubmed/chemical/Transforming Growth Factor beta,
http://linkedlifedata.com/resource/pubmed/chemical/Transforming Growth Factor beta1
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pubmed:status |
MEDLINE
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pubmed:month |
Dec
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pubmed:issn |
0741-5400
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
68
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
828-35
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pubmed:dateRevised |
2007-11-14
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pubmed:meshHeading |
pubmed-meshheading:11129650-Actins,
pubmed-meshheading:11129650-Adoptive Transfer,
pubmed-meshheading:11129650-Animals,
pubmed-meshheading:11129650-Antibodies, Monoclonal,
pubmed-meshheading:11129650-Autoimmune Diseases,
pubmed-meshheading:11129650-Biological Markers,
pubmed-meshheading:11129650-CD4-Positive T-Lymphocytes,
pubmed-meshheading:11129650-Cells, Cultured,
pubmed-meshheading:11129650-Collagen,
pubmed-meshheading:11129650-Fibroblasts,
pubmed-meshheading:11129650-Fibrosis,
pubmed-meshheading:11129650-Granuloma,
pubmed-meshheading:11129650-Immunization,
pubmed-meshheading:11129650-Interleukin-12,
pubmed-meshheading:11129650-Macrophages,
pubmed-meshheading:11129650-Mice,
pubmed-meshheading:11129650-Mice, Inbred CBA,
pubmed-meshheading:11129650-Mice, Inbred DBA,
pubmed-meshheading:11129650-Models, Animal,
pubmed-meshheading:11129650-RNA, Messenger,
pubmed-meshheading:11129650-Receptors, Interleukin-2,
pubmed-meshheading:11129650-T-Lymphocytes,
pubmed-meshheading:11129650-Thyroglobulin,
pubmed-meshheading:11129650-Thyroid Gland,
pubmed-meshheading:11129650-Thyroiditis, Autoimmune,
pubmed-meshheading:11129650-Thyroxine,
pubmed-meshheading:11129650-Transforming Growth Factor beta,
pubmed-meshheading:11129650-Transforming Growth Factor beta1
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pubmed:year |
2000
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pubmed:articleTitle |
Characterization of thyroid fibrosis in a murine model of granulomatous experimental autoimmune thyroiditis.
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pubmed:affiliation |
Department of Internal Medicine, University of Missouri, School of Medicine, Columbia 65212, USA.
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pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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