Source:http://linkedlifedata.com/resource/pubmed/id/11128025
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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
5
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pubmed:dateCreated |
2000-12-20
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pubmed:abstractText |
IL-18 is a cytokine with potent IFN-gamma inducing activities as well as an important mediator of Th1 polarized immune responses. In this study we demonstrated that IL-18 induces the concentration-dependent production of the proinflammatory mediators IFN-gamma, IL-6, and GM-CSF, but not the anti-inflammatory cytokine, IL-10 from peripheral blood lymphocytes in the presence of mitogen. Three neutralizing IL-18 monoclonal antibodies (MAbs) were investigated, one of which (2C10) inhibited IL-18 bioactivity with an IC50 of 0.1 nM and had a K(D) of 3.9 x 10(-11) M. A NOD/SCID mouse model engrafted with human peripheral blood lymphocytes was developed to test the in vivo efficacy of this MAb. The IFN-gamma production induced by LPS administration was inhibited approximately 90% by prior dosing of MAb 2C10. The therapeutic utility of a high-affinity IL-18 MAb may be of benefit in Th1-driven autoimmune diseases such as rheumatoid arthritis and Crohn's Disease, where elevated levels of IL-18 have been observed.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical | |
pubmed:status |
MEDLINE
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pubmed:month |
Oct
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pubmed:issn |
0272-457X
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
19
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
363-7
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pubmed:dateRevised |
2004-11-17
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pubmed:meshHeading |
pubmed-meshheading:11128025-Animals,
pubmed-meshheading:11128025-Antibodies, Monoclonal,
pubmed-meshheading:11128025-Antibody Specificity,
pubmed-meshheading:11128025-Cytokines,
pubmed-meshheading:11128025-Humans,
pubmed-meshheading:11128025-Immunoglobulin Isotypes,
pubmed-meshheading:11128025-Interleukin-18,
pubmed-meshheading:11128025-Leukocytes, Mononuclear,
pubmed-meshheading:11128025-Mice,
pubmed-meshheading:11128025-Mice, Inbred BALB C,
pubmed-meshheading:11128025-Mice, Inbred C57BL,
pubmed-meshheading:11128025-Mice, Inbred NOD,
pubmed-meshheading:11128025-Mice, SCID,
pubmed-meshheading:11128025-Neutralization Tests,
pubmed-meshheading:11128025-Rats,
pubmed-meshheading:11128025-Rats, Sprague-Dawley
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pubmed:year |
2000
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pubmed:articleTitle |
Characterization of the in vitro and in vivo activity of monoclonal antibodies to human IL-18.
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pubmed:affiliation |
Department of MCCS, SmithKline Beecham, Harlow, Essex, UK. Stephen_D_Holmes@sbphrd.com
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pubmed:publicationType |
Journal Article
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