Linked Life Data

11126370

Source:http://linkedlifedata.com/resource/pubmed/id/11126370

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
50
pubmed:dateCreated
2000-12-20
pubmed:abstractText
Myxoid and round-cell liposarcomas share the translocation t(12;16)(q13;p11) creating the TLS-CHOP fusion gene as a common genetic alteration. We previously reported several unique characteristics of genomic sequences around the breakpoints in the TLS and CHOP loci, and among them was the presence of consensus recognition motifs of Translin, a protein that associates with chromosomal translocations of lymphoid neoplasms. We further extended our search for Translin binding motifs in sequences adjacent to breakpoints and investigated whether Translin binds to these sequences in vitro by mobility-shift assay. Computer-assisted search found sequences highly homologous (>70%) with Translin binding motifs adjacent to the breakpoints in 10 out of 11 liposarcomas with the TLS-CHOP fusion genes. All of 13 oligonucleotides corresponding to the putative binding sequences in these cases bind to Hela cell extract and also recombinant Translin protein, although the binding affinity of each motif showed considerable differences. The DNA-protein complex formation was inhibited by non-labeled competitor or anti-Translin antibody, suggesting the specificity of the complex formation. Considering the high incidence and specific binding property, the presence of Translin binding motif may be one of the important determinants for the location of breakpoints in the TLS and CHOP genes in liposarcomas.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Nov
pubmed:issn
0950-9232
pubmed:author
pubmed:issnType
Print
pubmed:day
23
pubmed:volume
19
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
5821-5
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed-meshheading:11126370-Binding Sites, pubmed-meshheading:11126370-CCAAT-Enhancer-Binding Proteins, pubmed-meshheading:11126370-Chromosome Breakage, pubmed-meshheading:11126370-Chromosomes, Human, Pair 12, pubmed-meshheading:11126370-Chromosomes, Human, Pair 16, pubmed-meshheading:11126370-Consensus Sequence, pubmed-meshheading:11126370-DNA, Neoplasm, pubmed-meshheading:11126370-DNA-Binding Proteins, pubmed-meshheading:11126370-HeLa Cells, pubmed-meshheading:11126370-Humans, pubmed-meshheading:11126370-Liposarcoma, Myxoid, pubmed-meshheading:11126370-Oligonucleotides, pubmed-meshheading:11126370-Oncogene Proteins, Fusion, pubmed-meshheading:11126370-RNA-Binding Protein FUS, pubmed-meshheading:11126370-Substrate Specificity, pubmed-meshheading:11126370-Transcription Factor CHOP, pubmed-meshheading:11126370-Translocation, Genetic
pubmed:year
2000
pubmed:articleTitle
Translin binds to the sequences adjacent to the breakpoints of the TLS and CHOP genes in liposarcomas with translocation t(12;6).
pubmed:affiliation
Institute for Frontier Medical Sciences, Kyoto University, Shogoin, Japan.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't