Linked Life Data

11125315

Source:http://linkedlifedata.com/resource/pubmed/id/11125315

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
4
pubmed:dateCreated
2001-1-26
pubmed:abstractText
Testicular inflammation is classically observed in the pathogenesis of viral and bacterial infection or tumoral invasion. In these situations, leukocyte infiltration is generally encountered. GRO/KC (growth-related oncogene) and IP-10/mob-1 (IFN-gamma-inducible protein) are two CXC-chemokines which attract neutrophils and activated T lymphocytes, respectively, have been studied for their ability to participate to testicular inflammation (orchitis). In the present work, using Northern blot and immunocytochemistry, we aimed to investigate whether GRO/KC and IP-10/mob-1 are produced within the seminiferous tubules of the testis and if these chemokines are induced by a number of pro-inflammatory cytokines and lipopolysaccharides (LPS). Our results show that GRO/KC and IP-10/mob-1 mRNAs were never found in germ cells, whether they were stimulated or not. In contrast, GRO/KC mRNA was expressed by isolated peritubular cells when stimulated by interleukin-1 alpha and beta (IL-1 alpha and IL-1 beta) or LPS and to a lesser extent by tumor necrosis factor-alpha (TNF-alpha) and by Sertoli cells when the latter were stimulated by rIL-alpha and rIL-1 beta and to a lesser extent by TNF-alpha and LPS. Moreover, IP10/mob-1 transcripts were strongly induced in peritubular cells by interferon-alpha (IFN-alpha) and IFN-gamma, whereas, in isolated Sertoli cells, INF-alpha and TNF-alpha were the only potent inducers. The kinetics of GRO/KC and IP-10/mob-1 mRNA expression by peritubular and Sertoli cells (significant stimulation as early as 1 hour and 4 hours post-exposure to the stimuli, respectively) are compatible with the hypothesis of a rapid mobilisation of these cells in an inflammatory process. Moreover, the dose-dependent effects of pro-inflammatory cytokines to induce a chemokine response were compatible with a high sensitivity of peritubular and Sertoli cells in orchitis. In conclusion, this present study shows that 2 CXC-chemokines, GRO/KC and IP10/mob-1, are produced by testicular somatic cells of seminiferous tubules, strongly indicating a likely role of these chemokines in the accumulation of neutrophils and T lymphocytes during orchitis of various origins.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Dec
pubmed:issn
1148-5493
pubmed:author
pubmed:issnType
Print
pubmed:volume
11
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
690-8
pubmed:dateRevised
2008-11-21
pubmed:meshHeading
pubmed-meshheading:11125315-Animals, pubmed-meshheading:11125315-Blotting, Northern, pubmed-meshheading:11125315-Cells, Cultured, pubmed-meshheading:11125315-Chemokine CXCL10, pubmed-meshheading:11125315-Chemokines, pubmed-meshheading:11125315-Chemokines, CXC, pubmed-meshheading:11125315-Cytokines, pubmed-meshheading:11125315-Dose-Response Relationship, Immunologic, pubmed-meshheading:11125315-Germ Cells, pubmed-meshheading:11125315-Immunohistochemistry, pubmed-meshheading:11125315-Kinetics, pubmed-meshheading:11125315-Male, pubmed-meshheading:11125315-RNA, Messenger, pubmed-meshheading:11125315-Rats, pubmed-meshheading:11125315-Rats, Sprague-Dawley, pubmed-meshheading:11125315-Seminiferous Tubules, pubmed-meshheading:11125315-Sertoli Cells, pubmed-meshheading:11125315-Spermatogonia, pubmed-meshheading:11125315-Transcriptional Activation
pubmed:year
2000
pubmed:articleTitle
Expression and regulation of the CXC-chemokines, GRO/KC and IP-10/mob-1 in rat seminiferous tubules.
pubmed:affiliation
GERM-INSERM U. 435, Université de Rennes I, Campus de Beaulieu, 35042 Rennes Cedex, Bretagne, France.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't