Linked Life Data

11122450

Source:http://linkedlifedata.com/resource/pubmed/id/11122450

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
4
pubmed:dateCreated
2000-12-29
pubmed:abstractText
Chicken antibodies (immunoglobulin Y; IgY) to the alpha Gal epitope (galactose alpha-1,3-galactose) bind to alpha Gal antigens of mouse and porcine tissues and endothelial cells in vitro and block human anti-alpha Gal antibody binding, complement activation and antibody-dependent cell-mediated lysis mechanisms. The activities and toxicity of anti-alpha Gal IgY have not been tested in vivo. In this study, we tested the effects of multiple injections of affinity-purified anti-alpha Gal IgY (AP-IgY) in both wild-type (WT) and alpha-1,3-galactosyltransferase knockout (Gal KO) mice. WT and Gal KO mice were injected once, twice, three, or four times intravenously (i.v.) with AP-IgY and killed at 1 hr or 24 hr. Mice displayed no toxicity to four injections of AP-IgY. Heart, lung, liver, kidney, spleen and pancreatic tissue were evaluated using immunohistochemical techniques for the presence of the alpha Gal epitope using the GSI-B4 lectin, and for bound IgY, as well as mouse IgM and IgG. The binding of AP-IgY antibodies to the endothelium of WT mouse tissues was essentially identical to the pattern of binding of the GSI-B4 lectin after injection of WT mice and death at 1 hr. WT mice killed 24 hr after i.v. injection of AP-IgY showed little remaining bound IgY in their endothelia, indicating that IgY is cleared over that time period. We also evaluated the blood drawn at the time of death for the presence of anti-alpha Gal IgY, anti-IgY IgM and anti-IgY IgG by enzyme-linked immunosorbent assay. Anti-alpha Gal IgY was almost undetectable in WT mouse sera at all injection and killing times. In contrast, Gal KO mouse sera showed increasing anti-alpha Gal IgY levels until 24 hr after the fourth injection, when anti-alpha Gal IgY levels were almost undetectable. Anti-IgY IgM and IgG levels in WT and Gal KO mouse sera showed a typical increase in anti-IgY IgM 24 hr after the second injection (3 days after the first injection) and an increase in anti-IgY IgG 24 hr after the third injection (5 days after the first injection). These results show that IgY binds to alpha Gal epitopes in the WT mice and is cleared sometime over a 24-hr time period and that IgY is an expected immunogen in mice eliciting a rather typical anti-IgY IgM and IgG response.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/11122450-10431786, http://linkedlifedata.com/resource/pubmed/commentcorrection/11122450-10503781, http://linkedlifedata.com/resource/pubmed/commentcorrection/11122450-11653203, http://linkedlifedata.com/resource/pubmed/commentcorrection/11122450-1440638, http://linkedlifedata.com/resource/pubmed/commentcorrection/11122450-1908095, http://linkedlifedata.com/resource/pubmed/commentcorrection/11122450-7836412, http://linkedlifedata.com/resource/pubmed/commentcorrection/11122450-8560551, http://linkedlifedata.com/resource/pubmed/commentcorrection/11122450-8623267, http://linkedlifedata.com/resource/pubmed/commentcorrection/11122450-8623306, http://linkedlifedata.com/resource/pubmed/commentcorrection/11122450-8729877, http://linkedlifedata.com/resource/pubmed/commentcorrection/11122450-9361502, http://linkedlifedata.com/resource/pubmed/commentcorrection/11122450-9530963, http://linkedlifedata.com/resource/pubmed/commentcorrection/11122450-9665076, http://linkedlifedata.com/resource/pubmed/commentcorrection/11122450-9741457, http://linkedlifedata.com/resource/pubmed/commentcorrection/11122450-9771838
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Dec
pubmed:issn
0019-2805
pubmed:author
pubmed:issnType
Print
pubmed:volume
101
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
467-73
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed:year
2000
pubmed:articleTitle
Distribution of, and immune response to, chicken anti-alpha Gal immunoglobulin Y antibodies in wild-type and alpha Gal knockout mice.
pubmed:affiliation
Department of Surgery, Northwestern University Medical School, Chicago, IL 60611, USA.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't