|
rdf:type |
|
|
lifeskim:mentions |
|
|
pubmed:issue |
3
|
|
pubmed:dateCreated |
2000-12-26
|
|
pubmed:abstractText |
We have previously demonstrated that exogenous Nef protein induced activation of normal human T cells up-regulating IL-15 production by monocytes. Since HIV-1 infection results in the early impairment of immune functions we decided to evaluate if Nef is able to modulate the induction of a specific antibody response. Human peripheral blood mononuclear cells from healthy donors were induced in vitro to mount a specific antibody response to the Candida albicans antigen. We show that Nef inhibited, in a dose-dependent manner, the induction of the anti-C. albicans antibody response. The ability of an anti-Nef antibody to prevent such inhibition indicates that the effect was indeed Nef-specific. In the Nef-treated cultures an early increase of IL-15 production was observed and the addition of anti-IL-15 antibody abrogated the Nef-induced inhibitory effect. Moreover the addition of IL-15 to the cultures inhibited, as well as Nef, the induction of the specific antibody response. Thus, our results suggest that Nef may inhibit the induction of a specific antibody response by an early up-regulation of IL-15 production. A better comprehension of this phenomenon may be important for unravelling some aspects of the B cell defects in HIV infection.
|
|
pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/11122241-10388525,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11122241-1396975,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11122241-1527859,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11122241-1576656,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11122241-1694201,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11122241-1704397,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11122241-1706757,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11122241-1893630,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11122241-2014052,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11122241-2052609,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11122241-2265027,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11122241-2500954,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11122241-2556795,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11122241-2676560,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11122241-287037,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11122241-3058863,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11122241-7523571,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11122241-7529365,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11122241-7690375,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11122241-7724606,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11122241-7814861,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11122241-7821927,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11122241-8026467,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11122241-8086129,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11122241-8093551,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11122241-8168144,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11122241-8178155,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11122241-8579753,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11122241-8668345,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11122241-8804432,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11122241-8809126,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11122241-9097905,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11122241-9368628,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11122241-9743208
|
|
pubmed:language |
eng
|
|
pubmed:journal |
|
|
pubmed:citationSubset |
IM
|
|
pubmed:chemical |
|
|
pubmed:status |
MEDLINE
|
|
pubmed:month |
Dec
|
|
pubmed:issn |
0009-9104
|
|
pubmed:author |
|
|
pubmed:issnType |
Print
|
|
pubmed:volume |
122
|
|
pubmed:owner |
NLM
|
|
pubmed:authorsComplete |
Y
|
|
pubmed:pagination |
358-63
|
|
pubmed:dateRevised |
2009-11-18
|
|
pubmed:meshHeading |
pubmed-meshheading:11122241-Antibodies, Fungal,
pubmed-meshheading:11122241-Candida albicans,
pubmed-meshheading:11122241-Cells, Cultured,
pubmed-meshheading:11122241-Gene Products, nef,
pubmed-meshheading:11122241-HIV-1,
pubmed-meshheading:11122241-Humans,
pubmed-meshheading:11122241-Immunosuppression,
pubmed-meshheading:11122241-Interleukin-15,
pubmed-meshheading:11122241-Leukocytes, Mononuclear,
pubmed-meshheading:11122241-Up-Regulation,
pubmed-meshheading:11122241-nef Gene Products, Human Immunodeficiency Virus
|
|
pubmed:year |
2000
|
|
pubmed:articleTitle |
HIV-1 Nef protein inhibits the in vitro induction of a specific antibody response to Candida albicans by an early up-regulation of IL-15 production.
|
|
pubmed:affiliation |
Department of Immunology, Istituto Superiore di Sanità, Rome, Italy.
|
|
pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|
