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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
24
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pubmed:dateCreated |
2001-2-8
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pubmed:abstractText |
We report a new pathway of translation regulation that may operate in interferon-treated or virus-infected mammalian cells. This pathway is activated by P56, a protein whose synthesis is strongly induced by interferons or double-stranded RNA. Using a yeast two-hybrid screen, we identified the P48 subunit of the mammalian translation initiation factor eIF-3 as a protein that interacts with P56. The P56-P48 interaction was confirmed in human cells by co-immunoprecipitation assays and confocal microscopy. Gel filtration assays revealed that P56 binds to the large eIF-3 complex that contains P48. Purified recombinant P56 inhibited in vitro translation of reporter mRNAs in a dose-dependent fashion, and that inhibition was reversed by the addition of purified eIF-3. In vivo, expression of transfected P56 or induction of the endogenous P56 by interferon caused an inhibition of overall cellular protein synthesis and the synthesis of a transfected reporter protein. As expected, a P56 mutant that does not interact with P48 and eIF-3 failed to inhibit protein synthesis in vitro and in vivo.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/11118224-10400669,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11118224-10644362,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11118224-2118988,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11118224-2828026,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11118224-3097508,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11118224-3360121,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11118224-6186990,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11118224-7479831,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11118224-7545299,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11118224-7642650,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11118224-7679242,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11118224-7853537,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11118224-7896268,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11118224-8096514,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11118224-8391633,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11118224-8665893,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11118224-8688078,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11118224-8810342,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11118224-8862396,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11118224-9188575,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11118224-9207104,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11118224-9295280,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11118224-9398535,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11118224-9403073,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11118224-9482716,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11118224-9687506,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11118224-9759489,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11118224-9828129,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11118224-9861020
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Dec
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pubmed:issn |
0261-4189
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
15
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pubmed:volume |
19
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
6891-9
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:11118224-Animals,
pubmed-meshheading:11118224-Base Sequence,
pubmed-meshheading:11118224-Carrier Proteins,
pubmed-meshheading:11118224-Cloning, Molecular,
pubmed-meshheading:11118224-Eukaryotic Initiation Factor-3,
pubmed-meshheading:11118224-Fibrosarcoma,
pubmed-meshheading:11118224-Gene Expression Regulation,
pubmed-meshheading:11118224-Genes, Reporter,
pubmed-meshheading:11118224-HeLa Cells,
pubmed-meshheading:11118224-Humans,
pubmed-meshheading:11118224-Mammals,
pubmed-meshheading:11118224-Molecular Sequence Data,
pubmed-meshheading:11118224-Peptide Initiation Factors,
pubmed-meshheading:11118224-Protein Biosynthesis,
pubmed-meshheading:11118224-Protein Subunits,
pubmed-meshheading:11118224-RNA, Messenger,
pubmed-meshheading:11118224-Recombinant Proteins,
pubmed-meshheading:11118224-Saccharomyces cerevisiae,
pubmed-meshheading:11118224-Transfection,
pubmed-meshheading:11118224-Tumor Cells, Cultured
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pubmed:year |
2000
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pubmed:articleTitle |
A new pathway of translational regulation mediated by eukaryotic initiation factor 3.
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pubmed:affiliation |
Department of Molecular Biology, The Lerner Research Institute, Cleveland Clinic Foundation, 9500 Euclid Avenue, Cleveland, OH 44195, USA.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.
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