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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
24
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pubmed:dateCreated |
2001-2-8
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pubmed:abstractText |
We have shown previously that the TFIIIC1/TFIIIC1' fraction interacts specifically with the VA1 terminator regions to affect both termination and initiation/reinitiation of transcription by human RNA polymerase III. Here, we further purified the VA1 terminator-binding factor to apparent homogeneity and found, by peptide sequence analysis, that it belongs to the NF1 protein family. NF1 interacts specifically with the NF1-binding sites within the terminator regions of the VA1 gene and with two subunits (TFIIIC220 and TFIIIC110) of human TFIIIC2. Immunodepletion with anti-NF1 antibodies dramatically decreases transcription from the VA1 template in nuclear extract, and mutation at the NF1-binding site in the terminator region of the VA1 gene selectively affects multiple-round transcription (reinitiation of transcription) and termination. In addition, NF1 acts in conjunction with TFIIIC to promote accurate termination by RNA polymerase III on a C-tailed VA1 template.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/11118217-10567530,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11118217-10684922,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11118217-1447193,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11118217-1569098,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11118217-1694510,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11118217-1850908,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11118217-1996100,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11118217-2601722,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11118217-2720783,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11118217-2732244,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11118217-3398920,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11118217-3461465,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11118217-3473469,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11118217-3902472,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11118217-6194892,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11118217-6263489,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11118217-6328417,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11118217-6336326,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11118217-7540287,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11118217-7590749,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11118217-8026582,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11118217-8171010,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11118217-8423814,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11118217-8565070,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11118217-8581067,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11118217-8832903,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11118217-8943339,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11118217-9056636,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11118217-9171097,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11118217-9171375,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11118217-9433127,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11118217-9606195,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11118217-9660824,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11118217-9660958,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11118217-9742231,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11118217-9862998
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Dec
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pubmed:issn |
0261-4189
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
15
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pubmed:volume |
19
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
6823-32
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:11118217-Base Sequence,
pubmed-meshheading:11118217-Binding Sites,
pubmed-meshheading:11118217-Cell Nucleus,
pubmed-meshheading:11118217-DNA-Binding Proteins,
pubmed-meshheading:11118217-Humans,
pubmed-meshheading:11118217-Molecular Sequence Data,
pubmed-meshheading:11118217-NFI Transcription Factors,
pubmed-meshheading:11118217-Nuclear Proteins,
pubmed-meshheading:11118217-Oligonucleotide Probes,
pubmed-meshheading:11118217-RNA Polymerase III,
pubmed-meshheading:11118217-Templates, Genetic,
pubmed-meshheading:11118217-Terminator Regions, Genetic,
pubmed-meshheading:11118217-Transcription, Genetic,
pubmed-meshheading:11118217-Transcription Factors,
pubmed-meshheading:11118217-Transcription Factors, TFIII
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pubmed:year |
2000
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pubmed:articleTitle |
Nuclear factor 1 (NF1) affects accurate termination and multiple-round transcription by human RNA polymerase III.
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pubmed:affiliation |
The Laboratory of Biochemistry and Molecular Biology,The Rockefeller University, 1230 York Avenue, New York, NY 10021, USA.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.
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