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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
1
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pubmed:dateCreated |
1975-5-21
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pubmed:abstractText |
1. Glycopeptides isolated by exhaustive pronase digestion from the egg jelly coat of toad, Bufo vulgaris, were separated by DEAE-cellulose chromatography into two fractions. These fractions each gave a single band when they were subjected to electrophoresis on cellulose acetate strips. The glycopeptides behaved as a kind of "macroglycopeptide" on gel chromatography. 2. The glycopeptide fractions contained as sugar components galactosamine, glucosamine, galactose, fucose and N-acetylneuraminic acid. The amino acid composition was characterized by the presence of threonine and serine, which accounted for approximately 70% of the total amino acids. 3. Large amounts of galactosamine, threonine and serine were destroyed by treatment with alkali, indicating that the carbohydrate-peptide linkage in the glycopeptides is mostly an O-glycosidic bond between N-acetylgalactosamine and the hydroxy groups of threonine and serine.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical | |
pubmed:status |
MEDLINE
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pubmed:month |
Jan
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pubmed:issn |
0006-3002
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:day |
13
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pubmed:volume |
381
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
195-202
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pubmed:dateRevised |
2003-11-14
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pubmed:meshHeading |
pubmed-meshheading:1111585-Amino Acids,
pubmed-meshheading:1111585-Animals,
pubmed-meshheading:1111585-Bufonidae,
pubmed-meshheading:1111585-Chromatography,
pubmed-meshheading:1111585-Chromatography, DEAE-Cellulose,
pubmed-meshheading:1111585-Electrophoresis,
pubmed-meshheading:1111585-Female,
pubmed-meshheading:1111585-Glycopeptides,
pubmed-meshheading:1111585-Hexoses,
pubmed-meshheading:1111585-Ovum,
pubmed-meshheading:1111585-Protein Binding,
pubmed-meshheading:1111585-Sialic Acids
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pubmed:year |
1975
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pubmed:articleTitle |
Glycopeptides from the egg jelly coat of the toad Bufo vulgaris.
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pubmed:publicationType |
Journal Article
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