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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
Pt 1
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pubmed:dateCreated |
2001-1-26
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pubmed:abstractText |
Density-gradient centrifugation of bovine tracheal epithelial cell extracts revealed a 'high-density' (1.48 g/ml) sialic-acid-rich population as well as a 'low-density' (1.42 g/ml) one that reacted more strongly with a periodate-Schiff (PAS) assay. The sialic-acid-rich mucins were oligomeric molecules containing disulphide- bond-linked subunits and large glycosylated domains, whereas the PAS-reactive component seemed to be smaller and 'monomeric'. Only the 'high-density' population was secreted from cells cultured for 5 days on plastic or a collagen type 1, Matrigel or Vitrogen substrate. Release was less from cells grown on plastic than from those on a substrate and the amount was unaffected by increasing the thickness of the collagen layer. For cells grown on collagen, the amount of the sialic-acid-rich mucin increased over 10 days, whereas the PAS-reactive component was largely absent after 24 h, which was consistent with an initial release of stored PAS-reactive molecules and synthesis of the sialic-acid-rich mucins de novo. Both [(3)H]proline and [(35)S]sulphate were poorly incorporated into mucins detected with the chemical assays but molecules with a higher buoyant density than that of either of the previously identified species were labelled with [(35)S]sulphate. The [(35)S]sulphate-labelled material yielded large trypsin-resistant fragments and contained O-linked glycans but was not affected by digestion with chondroitin ABC lyase or heparan sulphate lyase, suggesting that it is a mucin rather than a proteoglycan. [(35)S]Sulphate is thus a poor marker for the major oligomeric mucins produced by bovine tracheal epithelial cells but the radiolabel is incorporated into a heavily labelled mucin-like component.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/11115395-10024507,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11115395-10229869,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11115395-10336486,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11115395-10463611,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11115395-10512748,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11115395-10630633,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11115395-10835316,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11115395-10880978,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11115395-1400449,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11115395-1449807,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11115395-1599454,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11115395-1616701,
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http://linkedlifedata.com/resource/pubmed/commentcorrection/11115395-3663189,
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http://linkedlifedata.com/resource/pubmed/commentcorrection/11115395-9761766
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jan
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pubmed:issn |
0264-6021
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
1
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pubmed:volume |
353
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
23-32
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:11115395-Alkylation,
pubmed-meshheading:11115395-Animals,
pubmed-meshheading:11115395-Cattle,
pubmed-meshheading:11115395-Cell Extracts,
pubmed-meshheading:11115395-Cells, Cultured,
pubmed-meshheading:11115395-Centrifugation, Isopycnic,
pubmed-meshheading:11115395-Centrifugation, Zonal,
pubmed-meshheading:11115395-Epithelial Cells,
pubmed-meshheading:11115395-Kinetics,
pubmed-meshheading:11115395-Molecular Weight,
pubmed-meshheading:11115395-Mucins,
pubmed-meshheading:11115395-N-Acetylneuraminic Acid,
pubmed-meshheading:11115395-Reducing Agents,
pubmed-meshheading:11115395-Trachea,
pubmed-meshheading:11115395-Trypsin
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pubmed:year |
2001
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pubmed:articleTitle |
Mucin biosynthesis and secretion in tracheal epithelial cells in primary culture.
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pubmed:affiliation |
Department of Cell and Molecular Biology, Section for Molecular Pathogenesis, Lund University, P.O. Box 94, S-22100 Lund, Sweden.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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