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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
48
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pubmed:dateCreated |
2000-12-20
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pubmed:abstractText |
B-Myb is a highly conserved member of the Myb transcription factor family. The primary transcript of the B-myb gene is spliced alternatively in two mRNAs which either contain or lack a sequence corresponding to the so-called exon 9A of c-myb. Recent studies showed that full-length B-Myb containing the exon 9A encoded amino acids is a cell cycle regulated transcription factor whose activity is stimulated by cyclin A/Cdk 2-dependent phosphorylation at the carboxyl-terminus of B-Myb. We have now investigated in more detail the transactivation potential of the shorter isoform of B-Myb lacking exon 9A. Here, we show that B-Myb lacking exon 9A has no transactivation activity even in the presence of cyclin A. This inactivity of the shorter isoform of B-Myb is not due an improper subcelluar localization. Our work suggests that B-Myb lacking exon 9A may act as an inhibitor for full-length B-Myb mediated transactivation. Furthermore, by analysing the transactivation potential of Gal4/B-Myb fusion proteins we have identified the amino-terminal part of the exon 9A as the principal transactivation domain of full-length B-Myb. The results presented here demonstrate that B-myb encodes both an activator and an inhibitor of transcription and, thus, reveal an additional level of regulation of B-Myb activity beside the known cyclin dependent mechanisms.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Cell Cycle Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Cyclin A,
http://linkedlifedata.com/resource/pubmed/chemical/DNA-Binding Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/MYBL2 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Mybl2 protein, mouse,
http://linkedlifedata.com/resource/pubmed/chemical/Protein Isoforms,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger,
http://linkedlifedata.com/resource/pubmed/chemical/Trans-Activators
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pubmed:status |
MEDLINE
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pubmed:month |
Nov
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pubmed:issn |
0950-9232
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
16
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pubmed:volume |
19
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
5428-34
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pubmed:dateRevised |
2008-11-21
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pubmed:meshHeading |
pubmed-meshheading:11114719-Alternative Splicing,
pubmed-meshheading:11114719-Amino Acid Sequence,
pubmed-meshheading:11114719-Animals,
pubmed-meshheading:11114719-Cell Cycle Proteins,
pubmed-meshheading:11114719-Cell Nucleus,
pubmed-meshheading:11114719-Chickens,
pubmed-meshheading:11114719-Conserved Sequence,
pubmed-meshheading:11114719-Cyclin A,
pubmed-meshheading:11114719-DNA-Binding Proteins,
pubmed-meshheading:11114719-Exons,
pubmed-meshheading:11114719-Genes, Reporter,
pubmed-meshheading:11114719-Humans,
pubmed-meshheading:11114719-Mice,
pubmed-meshheading:11114719-Molecular Sequence Data,
pubmed-meshheading:11114719-Peptide Mapping,
pubmed-meshheading:11114719-Protein Isoforms,
pubmed-meshheading:11114719-Protein Structure, Tertiary,
pubmed-meshheading:11114719-RNA, Messenger,
pubmed-meshheading:11114719-Sequence Homology, Amino Acid,
pubmed-meshheading:11114719-Trans-Activators,
pubmed-meshheading:11114719-Transcription, Genetic,
pubmed-meshheading:11114719-Transcriptional Activation
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pubmed:year |
2000
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pubmed:articleTitle |
An alternatively spliced isoform of B-Myb is a transcriptional inhibitor.
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pubmed:affiliation |
Institut für Biochemie, Westfälische-Wilhelms-Universität Münster, Germany.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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