Source:http://linkedlifedata.com/resource/pubmed/id/11114305
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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
12
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pubmed:dateCreated |
2001-3-20
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pubmed:abstractText |
LAP/C/EBPbeta is a member of the C/EBP family of transcription factors and contributes to the regulation of the acute phase response in hepatocytes. Here we show that IL-6 controls LAP/C/EBPbeta gene transcription and identify an IL-6 responsive element in the LAP/C/EBPbeta promoter, which contains no STAT3 DNA binding motif. However, luciferase reporter gene assays showed that STAT3 activation through the gp130 signal transducer molecule is involved in mediating IL-6-dependent LAP/C/EBPbeta transcription. Southwestern analysis indicated that IL-6 induces binding of a 68-kDa protein to the recently characterized CRE-like elements in the LAP/C/EBPbeta promoter. Transfection experiments using promoter constructs with mutated CRE-like elements revealed that these sites confer IL-6 responsiveness. Further analysis using STAT1/STAT3 chimeras identified specific domains of the protein that are required for the IL-6-dependent increase in LAP/C/EBPbeta gene transcription. Overexpression of the amino-terminal domain of STAT3 blocked the IL-6-mediated response, suggesting that the STAT3 amino terminus has an important function in IL-6-mediated transcription of the LAP/C/EBPbeta gene. These data lead to a model of how tethering STAT3 to a DNA-bound complex contributes to IL-6-dependent LAP/C/EBPbeta gene transcription. Our analysis describes a new mechanism by which STAT3 controls gene transcription and which has direct implication for the acute phase response in liver cells.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/CCAAT-Enhancer-Binding Protein-beta,
http://linkedlifedata.com/resource/pubmed/chemical/DNA Primers,
http://linkedlifedata.com/resource/pubmed/chemical/DNA-Binding Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Interleukin-6,
http://linkedlifedata.com/resource/pubmed/chemical/STAT3 Transcription Factor,
http://linkedlifedata.com/resource/pubmed/chemical/Stat3 protein, mouse,
http://linkedlifedata.com/resource/pubmed/chemical/Trans-Activators
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pubmed:status |
MEDLINE
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pubmed:month |
Mar
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pubmed:issn |
0021-9258
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:day |
23
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pubmed:volume |
276
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
9016-27
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pubmed:dateRevised |
2008-11-21
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pubmed:meshHeading |
pubmed-meshheading:11114305-Animals,
pubmed-meshheading:11114305-Base Sequence,
pubmed-meshheading:11114305-Binding Sites,
pubmed-meshheading:11114305-CCAAT-Enhancer-Binding Protein-beta,
pubmed-meshheading:11114305-DNA Primers,
pubmed-meshheading:11114305-DNA-Binding Proteins,
pubmed-meshheading:11114305-Gene Expression Regulation,
pubmed-meshheading:11114305-Immunoblotting,
pubmed-meshheading:11114305-Interleukin-6,
pubmed-meshheading:11114305-Mice,
pubmed-meshheading:11114305-Mice, Inbred C3H,
pubmed-meshheading:11114305-Promoter Regions, Genetic,
pubmed-meshheading:11114305-Protein Binding,
pubmed-meshheading:11114305-STAT3 Transcription Factor,
pubmed-meshheading:11114305-TATA Box,
pubmed-meshheading:11114305-Trans-Activators,
pubmed-meshheading:11114305-Transcription, Genetic
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pubmed:year |
2001
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pubmed:articleTitle |
Interleukin-6-induced tethering of STAT3 to the LAP/C/EBPbeta promoter suggests a new mechanism of transcriptional regulation by STAT3.
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pubmed:affiliation |
Department of Gastroenterology and Hepatology, Medizinische Hochschule Hannover, 30625 Hannover, Germany.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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