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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
1
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pubmed:dateCreated |
2001-1-25
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pubmed:abstractText |
Previous studies reported disruption of adenylyl cyclase (AC)-cyclic AMP (cAMP) signal transduction in brain of Alzheimer's disease (AD). We also demonstrated that basal and stimulated AC activities in the presence of calcium and calmodulin (Ca(2+)/CaM) were significantly decreased in AD parietal cortex. In the present study, we examined the amounts of Ca(2+)/CaM-sensitive types I and VIII AC, and Ca(2+)/CaM-insensitive type VII AC in the postmortem hippocampi from AD patients and age-matched controls using immunoblotting. The specificities of the anti-type VII and VIII AC antibodies were confirmed by preabsorption with their specific blocking peptides. We observed a significant decrease in the level of type I AC and a tendency to decrease in the level of type VIII AC in AD hippocampus. On the other hand, the level of type VII AC showed no alteration between AD and controls. A body of evidence from the studies with invertebrates and vertebrates suggests that types I and VIII AC may play an essential role in learning and memory. Our finding thus firstly demonstrated that a specific disruption of the Ca(2+)/CaM-sensitive AC isoforms is likely involved in the pathophysiology in AD hippocampus.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Dec
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pubmed:issn |
0006-3002
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
15
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pubmed:volume |
1535
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
60-8
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pubmed:dateRevised |
2009-9-3
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pubmed:meshHeading |
pubmed-meshheading:11113632-Adenylate Cyclase,
pubmed-meshheading:11113632-Age Factors,
pubmed-meshheading:11113632-Aged,
pubmed-meshheading:11113632-Aged, 80 and over,
pubmed-meshheading:11113632-Alzheimer Disease,
pubmed-meshheading:11113632-Antibodies,
pubmed-meshheading:11113632-Calcium,
pubmed-meshheading:11113632-Calmodulin,
pubmed-meshheading:11113632-Cyclic AMP,
pubmed-meshheading:11113632-Down-Regulation,
pubmed-meshheading:11113632-Electrophoresis, Polyacrylamide Gel,
pubmed-meshheading:11113632-Female,
pubmed-meshheading:11113632-Hippocampus,
pubmed-meshheading:11113632-Humans,
pubmed-meshheading:11113632-Immunoblotting,
pubmed-meshheading:11113632-Isoenzymes,
pubmed-meshheading:11113632-Male,
pubmed-meshheading:11113632-Middle Aged,
pubmed-meshheading:11113632-Neurons,
pubmed-meshheading:11113632-Postmortem Changes,
pubmed-meshheading:11113632-Time Factors
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pubmed:year |
2000
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pubmed:articleTitle |
Hippocampal level of neural specific adenylyl cyclase type I is decreased in Alzheimer's disease.
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pubmed:affiliation |
Department of Psychiatry and Psychotherapy, University of Würzburg, Germany. yamamoto@mail.uni-wuerzburg.de
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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