Linked Life Data

11112411

Source:http://linkedlifedata.com/resource/pubmed/id/11112411

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
2000-12-26
pubmed:abstractText
Gamma-linolenic acid (GLA, gamma-C18:3) is an essential fatty acid that plays a vital role in biological structures and cellular functions. Based on available sequence information and using polymerase chain reaction (PCR) technique, we cloned from the fungus Mucor rouxii the entire coding sequence of a delta(6)-desaturase enzyme, which is responsible for the transformation of linoleic acid into GLA. The deduced amino acid sequence of M. rouxii gene showed the highest homology with the plant delta(6)-desaturase. It comprises the characteristics of membrane-bound desaturases, including histidine-rich motifs and hydrophobic regions. A cytochrome b(5)-like domain was observed at the N-terminus. In addition to three conserved histidine-rich motifs, we found an additional histidine-rich motif, HKHHSH, downstream of the cytochrome b(5)-like domain, which is not present in previously cloned delta(6)-desaturase genes. Heterologous expression of the M. rouxii cDNA in Saccharomyces cerevisiae resulted in the synthesis and accumulation of GLA.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Dec
pubmed:issn
0006-291X
pubmed:author
pubmed:copyrightInfo
Copyright 2000 Academic Press.
pubmed:issnType
Print
pubmed:day
9
pubmed:volume
279
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
17-22
pubmed:dateRevised
2009-11-19
pubmed:meshHeading
pubmed:year
2000
pubmed:articleTitle
delta(6)-desaturase of Mucor rouxii with high similarity to plant delta(6)-desaturase and its heterologous expression in Saccharomyces cerevisiae.
pubmed:affiliation
Biochemical Engineering and Pilot Plant Research and Development Unit, National Center for Genetic Engineering and Biotechnology (BIOTEC), Bangkok 10400, Thailand.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't