|
rdf:type |
|
|
lifeskim:mentions |
|
|
pubmed:issue |
1-2
|
|
pubmed:dateCreated |
2001-1-4
|
|
pubmed:databankReference |
|
|
pubmed:abstractText |
Screening of chicken cDNA libraries has identified four distinct forms of topoisomerase IIalpha and beta cDNAs. Two of these, designated topo IIalpha-1 and topo IIbeta-1, were previously deposited in the database. The other two, topo IIalpha-2 and topo IIbeta-2, are novel variants that appear to be conserved between chicken and human. Topo IIalpha-2 encodes a protein with an additional 35 amino acids inserted after K321 of the chicken topo IIalpha-1 protein sequence. Topo IIbeta-2 encodes a protein missing 86 amino acids following V27 in the topo IIbeta-1 protein sequence. We have also detected several alternatively spliced forms of human topo IIalpha. One of these, topo IIalpha-3, appears to correspond to chicken topo IIalpha-2. The other two are novel. The existence of these alternatively spliced forms in mature cytoplasmic RNA was confirmed by RT-PCR in several cell lines. Interestingly, these alternatively spliced forms carry sites for post-translational modification, suggesting that they may be subject to differential regulation from the canonical forms. These results suggest that cells express a more complex repertoire of topo II isoforms than previously thought, raising the possibility that different forms of topo II may fulfil specialized functions in chromosome dynamics.
|
|
pubmed:language |
eng
|
|
pubmed:journal |
|
|
pubmed:citationSubset |
IM
|
|
pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, Neoplasm,
http://linkedlifedata.com/resource/pubmed/chemical/DNA,
http://linkedlifedata.com/resource/pubmed/chemical/DNA, Complementary,
http://linkedlifedata.com/resource/pubmed/chemical/DNA Topoisomerases, Type II,
http://linkedlifedata.com/resource/pubmed/chemical/DNA topoisomerase II alpha,
http://linkedlifedata.com/resource/pubmed/chemical/DNA topoisomerase II beta,
http://linkedlifedata.com/resource/pubmed/chemical/DNA-Binding Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Isoenzymes,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger
|
|
pubmed:status |
MEDLINE
|
|
pubmed:month |
Nov
|
|
pubmed:issn |
0378-1119
|
|
pubmed:author |
|
|
pubmed:issnType |
Print
|
|
pubmed:day |
27
|
|
pubmed:volume |
258
|
|
pubmed:owner |
NLM
|
|
pubmed:authorsComplete |
Y
|
|
pubmed:pagination |
183-92
|
|
pubmed:dateRevised |
2010-11-18
|
|
pubmed:meshHeading |
pubmed-meshheading:11111056-Alternative Splicing,
pubmed-meshheading:11111056-Amino Acid Sequence,
pubmed-meshheading:11111056-Animals,
pubmed-meshheading:11111056-Antigens, Neoplasm,
pubmed-meshheading:11111056-Base Sequence,
pubmed-meshheading:11111056-Chickens,
pubmed-meshheading:11111056-Conserved Sequence,
pubmed-meshheading:11111056-DNA,
pubmed-meshheading:11111056-DNA, Complementary,
pubmed-meshheading:11111056-DNA Topoisomerases, Type II,
pubmed-meshheading:11111056-DNA-Binding Proteins,
pubmed-meshheading:11111056-Genetic Variation,
pubmed-meshheading:11111056-HeLa Cells,
pubmed-meshheading:11111056-Humans,
pubmed-meshheading:11111056-Isoenzymes,
pubmed-meshheading:11111056-Molecular Sequence Data,
pubmed-meshheading:11111056-RNA, Messenger,
pubmed-meshheading:11111056-Reverse Transcriptase Polymerase Chain Reaction,
pubmed-meshheading:11111056-Sequence Alignment,
pubmed-meshheading:11111056-Sequence Analysis, DNA,
pubmed-meshheading:11111056-Sequence Homology, Amino Acid,
pubmed-meshheading:11111056-Tumor Cells, Cultured
|
|
pubmed:year |
2000
|
|
pubmed:articleTitle |
Two differentially spliced forms of topoisomerase IIalpha and beta mRNAs are conserved between birds and humans.
|
|
pubmed:affiliation |
Wellcome Centre for Cell Biology, Institute of Cell and Molecular Biology, University of Edinburgh, Edinburgh EH9 3JR, UK.
|
|
pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|
