Linked Life Data

11110798

Source:http://linkedlifedata.com/resource/pubmed/id/11110798

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
15
pubmed:dateCreated
2001-4-11
pubmed:databankReference
pubmed:abstractText
Eukaryotes possess multiple isoforms of the a subunit of the V(0) complex of vacuolar-type H(+)-ATPases (V-ATPases). Mutations in the V-ATPase a3 isoform have recently been shown to result in osteopetrosis, a fatal disease in humans, but no function has yet been ascribed to other isoforms. In Caenorhabditis elegans, the unc-32 mutant was originally isolated on the basis of its movement defect. We have isolated four new mutant alleles, the strongest of which is embryonic lethal. We show here that unc-32 corresponds to one of the four genes encoding a V-ATPase a subunit in the nematode, and we present their expression patterns and a molecular analysis of the gene family. unc-32 gives rise via alternative splicing to at least six transcripts. In the uncoordinated alleles, the transcript unc-32 B is affected, suggesting that it encodes an isoform that is targeted to synaptic vesicles of cholinergic neurons, where it would control neurotransmitter uptake or release. Other isoforms expressed widely during embryogenesis are mutated in the lethal alleles and would be involved in other acidic organelles. Our results indicate that V-ATPase a subunit genes are highly regulated and have tissue-specific function.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Apr
pubmed:issn
0021-9258
pubmed:author
pubmed:issnType
Print
pubmed:day
13
pubmed:volume
276
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
11913-21
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed-meshheading:11110798-Alleles, pubmed-meshheading:11110798-Alternative Splicing, pubmed-meshheading:11110798-Amino Acid Sequence, pubmed-meshheading:11110798-Animals, pubmed-meshheading:11110798-Base Sequence, pubmed-meshheading:11110798-Caenorhabditis elegans, pubmed-meshheading:11110798-Caenorhabditis elegans Proteins, pubmed-meshheading:11110798-DNA, Complementary, pubmed-meshheading:11110798-Evolution, Molecular, pubmed-meshheading:11110798-Genes, Lethal, pubmed-meshheading:11110798-Humans, pubmed-meshheading:11110798-Molecular Sequence Data, pubmed-meshheading:11110798-Mutation, pubmed-meshheading:11110798-Proton-Translocating ATPases, pubmed-meshheading:11110798-RNA, Messenger, pubmed-meshheading:11110798-Sequence Homology, Amino Acid, pubmed-meshheading:11110798-Vacuolar Proton-Translocating ATPases
pubmed:year
2001
pubmed:articleTitle
The Caenorhabditis elegans unc-32 gene encodes alternative forms of a vacuolar ATPase a subunit.
pubmed:affiliation
Laboratoire de Génétique et Physiologie du Développement, CNRS, INSERM, Université de la Méditerranée, Luminy Case 907, 13288 Marseille Cedex 9, France. pujol@ibdm.univ-mrs.fr
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't