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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
12
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pubmed:dateCreated |
2001-1-16
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pubmed:abstractText |
The scavenger receptor class B type I (SR-BI) mediates the selective uptake of cholesterol and cholesteryl ester (CE) from high density lipoprotein (HDL) into cells. The high expression in liver and steroidogenic tissues is compatible with a role of SR-BI in reverse cholesterol transport and steroid hormone synthesis. Ways of regulation thus far described include induction by trophic hormones via cAMP-activated protein kinase A (PKA) and the effects of cellular and plasma cholesterol. Here we show that vitamin E (vitE) has a major effect on the expression of SR-BI in rat liver and in a human hepatoma-derived cell line, HepG2. Feeding rats a vitE-depleted diet resulted in an 11-fold increase in the SR-BI protein level in liver tissue. This effect was readily reversed by feeding a vitE-enriched chow. In HepG2 cells, the expression of the human SR-BI homolog was reduced when the vitE content was increased by incubating the cells with vitE-loaded HDL or with phosphatidylcholine/vitE vesicles. The downregulation of human SR-BI (hSR-BI) was accompanied by a reduced level of protein kinase C (PKC) in the particulate cell fraction, and PKC inhibition decreased the expression of hSR-BI and the uptake of vitE and cholesterol from HDL. Our results are consistent with the view that the cellular level of vitE exerts a tight control over the expression of SR-BI. Furthermore, the inhibitory effect of vitE on PKC seems to be involved in the signaling pathway.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD36,
http://linkedlifedata.com/resource/pubmed/chemical/Lipoproteins, HDL,
http://linkedlifedata.com/resource/pubmed/chemical/Membrane Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Protein Kinase C,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Immunologic,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Lipoprotein,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Scavenger,
http://linkedlifedata.com/resource/pubmed/chemical/SCARB1 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Scarb1 protein, mouse,
http://linkedlifedata.com/resource/pubmed/chemical/Scarb1 protein, rat,
http://linkedlifedata.com/resource/pubmed/chemical/Scavenger Receptors, Class B,
http://linkedlifedata.com/resource/pubmed/chemical/Vitamin E
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pubmed:status |
MEDLINE
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pubmed:month |
Dec
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pubmed:issn |
0022-2275
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
41
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
2009-16
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pubmed:dateRevised |
2009-11-3
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pubmed:meshHeading |
pubmed-meshheading:11108734-Animals,
pubmed-meshheading:11108734-Antigens, CD36,
pubmed-meshheading:11108734-Down-Regulation,
pubmed-meshheading:11108734-Humans,
pubmed-meshheading:11108734-Lipoproteins, HDL,
pubmed-meshheading:11108734-Liver,
pubmed-meshheading:11108734-Male,
pubmed-meshheading:11108734-Membrane Proteins,
pubmed-meshheading:11108734-Protein Kinase C,
pubmed-meshheading:11108734-Rats,
pubmed-meshheading:11108734-Rats, Wistar,
pubmed-meshheading:11108734-Receptors, Immunologic,
pubmed-meshheading:11108734-Receptors, Lipoprotein,
pubmed-meshheading:11108734-Receptors, Scavenger,
pubmed-meshheading:11108734-Scavenger Receptors, Class B,
pubmed-meshheading:11108734-Signal Transduction,
pubmed-meshheading:11108734-Tumor Cells, Cultured,
pubmed-meshheading:11108734-Vitamin E,
pubmed-meshheading:11108734-Vitamin E Deficiency
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pubmed:year |
2000
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pubmed:articleTitle |
Regulation by vitamin E of the scavenger receptor BI in rat liver and HepG2 cells.
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pubmed:affiliation |
Department of Neonatology, University Hospital Charité, Humboldt University, 10098 Berlin, Germany.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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