Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
11
pubmed:dateCreated
2000-12-22
pubmed:abstractText
Introns in plant nuclear pre-mRNAs are highly enriched in U or U + A residues and this property is essential for efficient splicing. Moreover, 3'-untranslated regions (3'-UTRs) in plant pre-mRNAs are generally UA-rich and contain sequences that are important for the polyadenylation reaction. Here, we characterize two structurally related RNA-binding proteins (RBPs) from Nicotiana plumbaginifolia, referred to as RBP45 and RBP47, having specificity for oligouridylates. Both proteins contain three RBD-type RNA-binding domains and a glutamine-rich N-terminus, and share similarity with Nam8p, a protein associated with U1 snRNP in the yeast Saccharomyces cerevisiae. Deletion analysis of RBP45 and RBP47 indicated that the presence of at least two RBD are required for interaction with RNA and that domains other than RBD do not significantly contribute to binding. mRNAs for RBP45 and RBP47 and mRNAs encoding six related proteins in Arabidopsis thaliana are constitutively expressed in different plant organs. Indirect immunofluorescence and fractionation of cell extracts showed that RBP45 and RBP47 are localized in the nucleus. In vivo UV crosslinking experiments demonstrated their association with the nuclear poly(A)+ RNA. In contrast to UBP1, another oligouridylate-binding nuclear three-RBD protein of N. plumbaginifolia (Lambermon et al., EMBO J, 2000, 19:1638-1649), RBP45 and RBP47 do not stimulate mRNA splicing and accumulation when transiently overexpressed in protoplasts. Properties of RBP45 and RBP47 suggest they represent hnRNP-proteins participating in still undefined steps of pre-mRNA maturation in plant cell nuclei.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/11105760-10072385, http://linkedlifedata.com/resource/pubmed/commentcorrection/11105760-10371034, http://linkedlifedata.com/resource/pubmed/commentcorrection/11105760-10395553, http://linkedlifedata.com/resource/pubmed/commentcorrection/11105760-10395560, http://linkedlifedata.com/resource/pubmed/commentcorrection/11105760-10500099, http://linkedlifedata.com/resource/pubmed/commentcorrection/11105760-10688363, http://linkedlifedata.com/resource/pubmed/commentcorrection/11105760-10694877, http://linkedlifedata.com/resource/pubmed/commentcorrection/11105760-10733580, http://linkedlifedata.com/resource/pubmed/commentcorrection/11105760-10740297, http://linkedlifedata.com/resource/pubmed/commentcorrection/11105760-10747031, http://linkedlifedata.com/resource/pubmed/commentcorrection/11105760-1346756, http://linkedlifedata.com/resource/pubmed/commentcorrection/11105760-1406585, http://linkedlifedata.com/resource/pubmed/commentcorrection/11105760-1655278, http://linkedlifedata.com/resource/pubmed/commentcorrection/11105760-1696344, http://linkedlifedata.com/resource/pubmed/commentcorrection/11105760-2758463, http://linkedlifedata.com/resource/pubmed/commentcorrection/11105760-3144044, http://linkedlifedata.com/resource/pubmed/commentcorrection/11105760-3724460, http://linkedlifedata.com/resource/pubmed/commentcorrection/11105760-3724473, http://linkedlifedata.com/resource/pubmed/commentcorrection/11105760-7680064, http://linkedlifedata.com/resource/pubmed/commentcorrection/11105760-7753842, http://linkedlifedata.com/resource/pubmed/commentcorrection/11105760-7768997, http://linkedlifedata.com/resource/pubmed/commentcorrection/11105760-7926791, http://linkedlifedata.com/resource/pubmed/commentcorrection/11105760-7962083, http://linkedlifedata.com/resource/pubmed/commentcorrection/11105760-8158249, http://linkedlifedata.com/resource/pubmed/commentcorrection/11105760-8336697, http://linkedlifedata.com/resource/pubmed/commentcorrection/11105760-8413212, http://linkedlifedata.com/resource/pubmed/commentcorrection/11105760-8413213, http://linkedlifedata.com/resource/pubmed/commentcorrection/11105760-8441378, http://linkedlifedata.com/resource/pubmed/commentcorrection/11105760-8576255, http://linkedlifedata.com/resource/pubmed/commentcorrection/11105760-8604302, http://linkedlifedata.com/resource/pubmed/commentcorrection/11105760-8626503, http://linkedlifedata.com/resource/pubmed/commentcorrection/11105760-8704159, http://linkedlifedata.com/resource/pubmed/commentcorrection/11105760-8772383, http://linkedlifedata.com/resource/pubmed/commentcorrection/11105760-8811184, http://linkedlifedata.com/resource/pubmed/commentcorrection/11105760-8824440, http://linkedlifedata.com/resource/pubmed/commentcorrection/11105760-8831291, http://linkedlifedata.com/resource/pubmed/commentcorrection/11105760-8871565, http://linkedlifedata.com/resource/pubmed/commentcorrection/11105760-8980472, http://linkedlifedata.com/resource/pubmed/commentcorrection/11105760-8980473, http://linkedlifedata.com/resource/pubmed/commentcorrection/11105760-8994828, http://linkedlifedata.com/resource/pubmed/commentcorrection/11105760-9056715, http://linkedlifedata.com/resource/pubmed/commentcorrection/11105760-9111328, http://linkedlifedata.com/resource/pubmed/commentcorrection/11105760-9148938, http://linkedlifedata.com/resource/pubmed/commentcorrection/11105760-9159082, http://linkedlifedata.com/resource/pubmed/commentcorrection/11105760-9229110, http://linkedlifedata.com/resource/pubmed/commentcorrection/11105760-9263462, http://linkedlifedata.com/resource/pubmed/commentcorrection/11105760-9311730, http://linkedlifedata.com/resource/pubmed/commentcorrection/11105760-9334319, http://linkedlifedata.com/resource/pubmed/commentcorrection/11105760-9349252, http://linkedlifedata.com/resource/pubmed/commentcorrection/11105760-9353246, http://linkedlifedata.com/resource/pubmed/commentcorrection/11105760-9418039, http://linkedlifedata.com/resource/pubmed/commentcorrection/11105760-9482885, http://linkedlifedata.com/resource/pubmed/commentcorrection/11105760-9484452, http://linkedlifedata.com/resource/pubmed/commentcorrection/11105760-9497373, http://linkedlifedata.com/resource/pubmed/commentcorrection/11105760-9628880, http://linkedlifedata.com/resource/pubmed/commentcorrection/11105760-9628881, http://linkedlifedata.com/resource/pubmed/commentcorrection/11105760-9630243, http://linkedlifedata.com/resource/pubmed/commentcorrection/11105760-9630245, http://linkedlifedata.com/resource/pubmed/commentcorrection/11105760-9724692, http://linkedlifedata.com/resource/pubmed/commentcorrection/11105760-9724693, http://linkedlifedata.com/resource/pubmed/commentcorrection/11105760-9857200, http://linkedlifedata.com/resource/pubmed/commentcorrection/11105760-9890998, http://linkedlifedata.com/resource/pubmed/commentcorrection/11105760-9925643, http://linkedlifedata.com/resource/pubmed/commentcorrection/11105760-9928482
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Nov
pubmed:issn
1355-8382
pubmed:author
pubmed:issnType
Print
pubmed:volume
6
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1610-24
pubmed:dateRevised
2010-9-14
pubmed:meshHeading
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