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rdf:type |
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lifeskim:mentions |
umls-concept:C0003316,
umls-concept:C0027651,
umls-concept:C0030956,
umls-concept:C0042210,
umls-concept:C0063356,
umls-concept:C0178539,
umls-concept:C0205263,
umls-concept:C0206243,
umls-concept:C1282910,
umls-concept:C1545588,
umls-concept:C1749881
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pubmed:issue |
22
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pubmed:dateCreated |
2000-12-4
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pubmed:abstractText |
Efficient loading of MHC class II molecules with a T helper epitope of choice can be achieved through genetic exchange of the MHC class II-associated invariant chain peptide (CLIP) sequence with a sequence encoding the helper peptide. We have now used this method to engineer a cellular vaccine that continuously expresses a tumor-specific helper epitope in a defined costimulatory context. We provide evidence (a) that this cellular vaccine induces peptide-specific helper T cells in vivo that are functional in protecting mice from challenge with a highly aggressive tumor, (b) that this vaccine can directly prime tumor-specific helper T cells in vivo, and (c) that this cellular vaccine is superior compared with similar cells loaded with synthetic T helper peptide in inducing tumor protection. In conclusion, cellular vaccines for activation of antigen-specific helper T cells can be greatly improved by the introduction of invariant chain constructs containing a T helper epitope by class II-associated invariant chain peptide exchange.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, Differentiation...,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, Neoplasm,
http://linkedlifedata.com/resource/pubmed/chemical/Cancer Vaccines,
http://linkedlifedata.com/resource/pubmed/chemical/Epitopes, T-Lymphocyte,
http://linkedlifedata.com/resource/pubmed/chemical/Histocompatibility Antigens Class II,
http://linkedlifedata.com/resource/pubmed/chemical/Retroviridae Proteins, Oncogenic,
http://linkedlifedata.com/resource/pubmed/chemical/Viral Envelope Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/invariant chain
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pubmed:status |
MEDLINE
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pubmed:month |
Nov
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pubmed:issn |
0008-5472
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
15
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pubmed:volume |
60
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
6427-33
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:11103809-Amino Acid Sequence,
pubmed-meshheading:11103809-Animals,
pubmed-meshheading:11103809-Antigen Presentation,
pubmed-meshheading:11103809-Antigen-Presenting Cells,
pubmed-meshheading:11103809-Antigens, Differentiation, B-Lymphocyte,
pubmed-meshheading:11103809-Antigens, Neoplasm,
pubmed-meshheading:11103809-Cancer Vaccines,
pubmed-meshheading:11103809-Epitopes, T-Lymphocyte,
pubmed-meshheading:11103809-Female,
pubmed-meshheading:11103809-Histocompatibility Antigens Class II,
pubmed-meshheading:11103809-Lymphocyte Activation,
pubmed-meshheading:11103809-Lymphoma, T-Cell,
pubmed-meshheading:11103809-Mice,
pubmed-meshheading:11103809-Mice, Inbred BALB C,
pubmed-meshheading:11103809-Mice, Inbred C57BL,
pubmed-meshheading:11103809-Molecular Sequence Data,
pubmed-meshheading:11103809-Retroviridae Proteins, Oncogenic,
pubmed-meshheading:11103809-T-Lymphocytes, Helper-Inducer,
pubmed-meshheading:11103809-Transfection,
pubmed-meshheading:11103809-Vaccination,
pubmed-meshheading:11103809-Viral Envelope Proteins
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pubmed:year |
2000
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pubmed:articleTitle |
Superior tumor protection induced by a cellular vaccine carrying a tumor-specific T helper epitope by genetic exchange of the class II-associated invariant chain peptide.
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pubmed:affiliation |
Department of Immunohematology and Blood Transfusion, Leiden University Medical Center, The Netherlands. jv228@cam.ac.uk
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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