Linked Life Data

11103774

Source:http://linkedlifedata.com/resource/pubmed/id/11103774

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pubmed-article:11103774pubmed:abstractTextA t(9;11)(p22;p15) chromosomal translocation was identified in an adult patient with de novo acute myelogenous leukemia. Fluorescence in situ hybridization and Southern blot analysis mapped the 11p15 break-point to the NUP98 gene. Using 3' rapid amplification of cDNA ends, we have identified a chimeric mRNA that fused the NUP98 FXFG repeats in frame to the COOH-terminal portion of the gene encoding the transcriptional coactivators p52 and p75, also known as lens epithelium-derived growth factor (LEDGF). As expected, both NUP98-p52 and NUP98-p75 (LEDGF) chimeric mRNAs were detected by reverse transcription-PCR; however, the reciprocal p52/p75 (LEDGF)-NUP98 fusion mRNA was not detected. Our results demonstrate that this is the most 5' NUP98 fusion reported and reveal a previously unrecognized genetic target, the gene encoding p52/p75 (LEDGF).lld:pubmed
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pubmed-article:11103774pubmed:articleTitlet(9;11)(p22;p15) in acute myeloid leukemia results in a fusion between NUP98 and the gene encoding transcriptional coactivators p52 and p75-lens epithelium-derived growth factor (LEDGF).lld:pubmed
pubmed-article:11103774pubmed:affiliationDepurtment of Medicine, Roswell Park Cancer Institute, Buffalo, New York 14263, USA. harisha@waushosp.orglld:pubmed
pubmed-article:11103774pubmed:publicationTypeJournal Articlelld:pubmed
pubmed-article:11103774pubmed:publicationTypeResearch Support, U.S. Gov't, P.H.S.lld:pubmed
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