Source:http://linkedlifedata.com/resource/pubmed/id/11101695
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
12
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| pubmed:dateCreated |
2001-1-16
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| pubmed:abstractText |
RL95-2 is a human uterine epithelial cell line that exhibits adhesion competence on its apical surface for trophoblast-like JAR cells. Using confocal microscopy and an adhesion assay we have found that changes in intracellular free calcium ([Ca(2+)](i)) in RL95-2 cells are involved in binding of JAR spheroids. Impact of spheroids upon, and movement of spheroids across, monolayers of RL95-2 cells produced a transient increase in [Ca(2+)](i). Pretreatment of RL95-2 cells with the Ca(2+) channel inhibitor, diltiazem, reduced the [Ca(2+)](i) increase. Interestingly, resting of JAR spheroids on RL95-2 cells caused no detectable alterations in [Ca(2+)](i) although cell-cell bonds were formed during prolonged contact. However, separation of established bonds did produce an increase in [Ca(2+)](i) which could be reduced by the Ca(2+) channel blocker, SKF-96365, but not by diltiazem. SKF-96365 also reduced adhesion of JAR spheroids to RL95-2 cells. In all experiments, the increase in [Ca(2+)](i) was due to influx from the external medium, as it could be blocked both by removing extracellular Ca(2+) and by nickel. These results suggest that the plasma membrane of uterine RL95-2 cells contains two types of Ca(2+) channels that are involved in trophoblast adhesion, i.e. diltiazem-sensitive channels contributing to initiation of JAR cell binding and SKF-96365-sensitive channels participating in a feedback loop that controls the balance of bonds.
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| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
IM
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| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/1-(2-(3-(4-methoxyphenyl)propoxy)-4-...,
http://linkedlifedata.com/resource/pubmed/chemical/Calcium,
http://linkedlifedata.com/resource/pubmed/chemical/Calcium Channel Blockers,
http://linkedlifedata.com/resource/pubmed/chemical/Diltiazem,
http://linkedlifedata.com/resource/pubmed/chemical/Imidazoles
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| pubmed:status |
MEDLINE
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| pubmed:month |
Dec
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| pubmed:issn |
1360-9947
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| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
6
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| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
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| pubmed:pagination |
1119-30
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| pubmed:dateRevised |
2004-11-17
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| pubmed:meshHeading |
pubmed-meshheading:11101695-Calcium,
pubmed-meshheading:11101695-Calcium Channel Blockers,
pubmed-meshheading:11101695-Calcium Signaling,
pubmed-meshheading:11101695-Cell Adhesion,
pubmed-meshheading:11101695-Cell Communication,
pubmed-meshheading:11101695-Cell Line,
pubmed-meshheading:11101695-Cell Movement,
pubmed-meshheading:11101695-Diltiazem,
pubmed-meshheading:11101695-Embryo Implantation,
pubmed-meshheading:11101695-Endometrium,
pubmed-meshheading:11101695-Epithelial Cells,
pubmed-meshheading:11101695-Female,
pubmed-meshheading:11101695-Humans,
pubmed-meshheading:11101695-Imidazoles,
pubmed-meshheading:11101695-Models, Biological,
pubmed-meshheading:11101695-Spheroids, Cellular,
pubmed-meshheading:11101695-Trophoblasts,
pubmed-meshheading:11101695-Tumor Cells, Cultured,
pubmed-meshheading:11101695-Uterus
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| pubmed:year |
2000
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| pubmed:articleTitle |
Calcium influx in human uterine epithelial RL95-2 cells triggers adhesiveness for trophoblast-like cells. Model studies on signalling events during embryo implantation.
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| pubmed:affiliation |
Max-Planck Institut für molekulare Physiologie, D-44227 Dortmund, Germany.
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| pubmed:publicationType |
Journal Article
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