Linked Life Data

11101682

Source:http://linkedlifedata.com/resource/pubmed/id/11101682

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:dateCreated
2000-12-29
pubmed:abstractText
The absorption spectrum of factor F(420) changes depending on the pH and the redox state of the cytoplasm. Specific wavelengths were used to calibrate absorption changes to allow the measurement of changes in the cytoplasmic pH in Methanobacterium thermoautotrophicum. Upon a hydrogen pulse, a rapid efflux of protons was observed. Under these energized conditions, the DeltapH amounts to 0.2-0.4 pH units at pH 6.6, and 0.6-0.8 pH units at pH 6.0. It decays within 10-20 s. In parallel, a sodium gradient is formed which has a slightly longer lifetime. Both DeltapH and DeltaPsi contribute to the proton-motive force present during methanogenesis. The energy-conversion rate, as indicated by the decay of the energized state of the cell, is fastest under growth conditions, i.e. at pH 6.9 and at a temperature of 58 degrees C.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Dec
pubmed:issn
1350-0872
pubmed:author
pubmed:issnType
Print
pubmed:volume
146 Pt 12
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
3245-50
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
2000
pubmed:articleTitle
Continuous monitoring of the cytoplasmic pH in Methanobacterium thermoautotrophicum using the intracellular factor F(420) as indicator.
pubmed:affiliation
Institute of Plant Biology, University of Zürich, Zollikerstrasse 107, CH-8008 Zürich, Switzerland.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't