Linked Life Data

11101679

Source:http://linkedlifedata.com/resource/pubmed/id/11101679

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:dateCreated
2000-12-29
pubmed:databankReference
pubmed:abstractText
Salmonella enterica is a facultative intracellular pathogen that is capable of causing disease in a range of hosts. Although human salmonellosis is frequently associated with consumption of contaminated poultry and eggs, and the serotypes Salmonella gallinarum and Salmonella pullorum are important world-wide pathogens of poultry, little is understood of the mechanisms of pathogenesis of Salmonella in the chicken. Type III secretion systems play a key role in host cell invasiveness and trigger the production of pro-inflammatory cytokines during invasion of mammalian hosts. This results in a polymorphonuclear cell influx that contributes to the resulting enteritis. In this study, a chicken primary cell culture model was used to investigate the cytokine responses to entry by the broad host range serotypes S. enteritidis and S. typhimurium, and the host specific serotype S. gallinarum, which rarely causes disease outside its main host, the chicken. The cytokines interleukin (IL)-1ss, IL-2, IL-6 and interferon (IFN)-gamma were measured by quantitative RT-PCR, and production of IL-6 and IFN-gamma was also determined through bioassays. All serotypes were invasive and had little effect on the production of IFN-gamma compared with non-infected cells; S. enteritidis invasion caused a slight down-regulation of IL-2 production. For IL-1ss production, infection with S. typhimurium had little effect, whilst infection with S. gallinarum or S. enteritidis caused a reduction in IL-1ss mRNA levels. Invasion of S. typhimurium and S. enteritidis caused an eight- to tenfold increase in production of the pro-inflammatory cytokine IL-6, whilst invasion by S. gallinarum caused no increase. These findings correlate with the pathogenesis of Salmonella in poultry. S. typhimurium and S. enteritidis invasion produces a strong inflammatory response, that may limit the spread of Salmonella largely to the gut, whilst S. gallinarum does not induce an inflammatory response and may not be limited by the immune system, leading to the severe systemic disease fowl typhoid.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Dec
pubmed:issn
1350-0872
pubmed:author
pubmed:issnType
Print
pubmed:volume
146 Pt 12
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
3217-26
pubmed:dateRevised
2008-11-21
pubmed:meshHeading
pubmed-meshheading:11101679-Animals, pubmed-meshheading:11101679-Biological Assay, pubmed-meshheading:11101679-Cell Line, pubmed-meshheading:11101679-Cells, Cultured, pubmed-meshheading:11101679-Chickens, pubmed-meshheading:11101679-Cytokines, pubmed-meshheading:11101679-Interferon-gamma, pubmed-meshheading:11101679-Interleukin-1, pubmed-meshheading:11101679-Interleukin-2, pubmed-meshheading:11101679-Interleukin-6, pubmed-meshheading:11101679-Kidney, pubmed-meshheading:11101679-Macrophage Activation, pubmed-meshheading:11101679-Macrophages, pubmed-meshheading:11101679-Molecular Sequence Data, pubmed-meshheading:11101679-Reverse Transcriptase Polymerase Chain Reaction, pubmed-meshheading:11101679-Salmonella, pubmed-meshheading:11101679-Salmonella enteritidis, pubmed-meshheading:11101679-Salmonella typhimurium
pubmed:year
2000
pubmed:articleTitle
Differential cytokine expression in avian cells in response to invasion by Salmonella typhimurium, Salmonella enteritidis and Salmonella gallinarum.
pubmed:affiliation
Institute for Animal Health, Compton, Berkshire RG20 7NN, UK Department of Animal and Food Sciences, University of Delaware, 531 South College Avenue, Newark, DE 19717-1303, USA.
pubmed:publicationType
Journal Article, Comparative Study, Research Support, Non-U.S. Gov't