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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
12
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pubmed:dateCreated |
2001-1-11
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pubmed:abstractText |
We generated a new monoclonal antibody (MAb), F7.2.38, by immunizing mice with CD3varepsilongammadelta/CD3omega complexes purified from human T-cells by OKT3 MAb-Sepharose affinity chromatography. Immunoprecipitation experiments and Western blotting analysis showed that MAb F7.2.38 recognized the CD3varepsilon chain in CD3varepsilon cDNA-transfected FOX B-cells and in various T-cell lines. Using flow cytometry on permeabilized or intact cells, the epitope was found to be located in the cytoplasmic tail of the CD3varepsilon chain. Immunohistochemical staining on paraffin-embedded sections showed that the reactivity of MAb F7.2.38 was comparable to that of the commercially available anti-CD3varepsilon polyclonal antibody. Of the 52 well-characterized T-cell lymphomas, 41 were positive for F7. 2.38 (79%), whereas all 37 B-cell lymphomas and 69 non-lymphoid tumors were unreactive. This new anti-CD3varepsilon antibody would be particularly useful for phenotyping T-cell lymphomas on routinely processed paraffin-embedded tissue sections.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antibodies, Monoclonal,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD3,
http://linkedlifedata.com/resource/pubmed/chemical/CD3E protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Epitopes,
http://linkedlifedata.com/resource/pubmed/chemical/Fixatives,
http://linkedlifedata.com/resource/pubmed/chemical/Formaldehyde,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Antigen, T-Cell
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pubmed:status |
MEDLINE
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pubmed:month |
Dec
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pubmed:issn |
0022-1554
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
48
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1609-16
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:11101629-Animals,
pubmed-meshheading:11101629-Antibodies, Monoclonal,
pubmed-meshheading:11101629-Antigens, CD3,
pubmed-meshheading:11101629-Blotting, Western,
pubmed-meshheading:11101629-Cell Line,
pubmed-meshheading:11101629-Epitopes,
pubmed-meshheading:11101629-Fixatives,
pubmed-meshheading:11101629-Flow Cytometry,
pubmed-meshheading:11101629-Formaldehyde,
pubmed-meshheading:11101629-Frozen Sections,
pubmed-meshheading:11101629-Hodgkin Disease,
pubmed-meshheading:11101629-Humans,
pubmed-meshheading:11101629-Lymphoma, Non-Hodgkin,
pubmed-meshheading:11101629-Mice,
pubmed-meshheading:11101629-Paraffin Embedding,
pubmed-meshheading:11101629-Precipitin Tests,
pubmed-meshheading:11101629-Rabbits,
pubmed-meshheading:11101629-Receptors, Antigen, T-Cell,
pubmed-meshheading:11101629-T-Lymphocytes,
pubmed-meshheading:11101629-Tissue Fixation
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pubmed:year |
2000
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pubmed:articleTitle |
A new monoclonal anti-CD3epsilon antibody reactive on paraffin sections.
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pubmed:affiliation |
Unité de Physiopathologie Cellulaire et Moléculaire, CNRS-UPR 2163, Institut Claude de Preval, IFR 30, Chu de Purpan, Toulouse, France.
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pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, Non-U.S. Gov't
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