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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
12
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pubmed:dateCreated |
2001-1-3
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pubmed:abstractText |
We have isolated piroplasms from a patient who developed the first case of human babesiosis in Japan by using NOD/shi-scid mice whose circulating erythrocytes (RBCs) had been replaced with human RBCs (hu-RBC-SCID mice). Following inoculation of the patient's blood specimen into hu-RBC-SCID mice, parasites proliferated within the human RBCs in the mice, resulting in a high level of parasitemia. Parasite DNA was prepared from blood samples of the patient and the mice, and the nuclear small-subunit rRNA gene (rDNA) was amplified and sequenced. Both DNA samples gave rise to identical sequences which showed the highest degree of homology (99.2%) with the Babesia microti rDNA. Because the patient had received a blood transfusion before the onset of babesiosis, we investigated the eight donors who were involved. Their archived blood samples were analyzed for specific antibody and parasite DNA; only a single donor was found to be positive by both tests, and the parasite rDNA sequence from the donor coincided with that derived from the patient. The donor's serum exhibited a high antibody titer against the isolate from the patient, whereas it exhibited only a weak cross-reaction against B. microti strains isolated in the United States. We conclude that the first Japanese babesiosis case occurred due to a blood transfusion and that the etiological agent is an indigenous Japanese parasite which may be a geographical variant of B. microti. Our results also demonstrated the usefulness of hu-RBC-SCID mice for isolation of parasites from humans and for maintenance of the parasite infectivity for human RBCs.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/11101588-10078490,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11101588-10618117,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11101588-11020989,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11101588-1279996,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11101588-1323922,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11101588-1500517,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11101588-1591615,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11101588-1635038,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11101588-271968,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11101588-3224833,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11101588-3447015,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11101588-4132544,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11101588-480073,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11101588-4994129,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11101588-7180791,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11101588-7200341,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11101588-763361,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11101588-7696408,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11101588-7760012,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11101588-7816065,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11101588-781840,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11101588-8169390,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11101588-8262654,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11101588-8328736,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11101588-8410556,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11101588-8542992,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11101588-8551053,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11101588-8578814,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11101588-8607592,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11101588-8637139,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11101588-8780464,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11101588-8904446,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11101588-9003614,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11101588-9617892,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11101588-9683900,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11101588-9770629
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Dec
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pubmed:issn |
0095-1137
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
38
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
4511-6
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:11101588-Animals,
pubmed-meshheading:11101588-Babesia,
pubmed-meshheading:11101588-Babesiosis,
pubmed-meshheading:11101588-Blood Transfusion,
pubmed-meshheading:11101588-Erythrocytes,
pubmed-meshheading:11101588-Humans,
pubmed-meshheading:11101588-Mice,
pubmed-meshheading:11101588-Mice, Inbred NOD,
pubmed-meshheading:11101588-Mice, SCID,
pubmed-meshheading:11101588-Phylogeny,
pubmed-meshheading:11101588-RNA, Ribosomal
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pubmed:year |
2000
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pubmed:articleTitle |
Transfusion-acquired, autochthonous human babesiosis in Japan: isolation of Babesia microti-like parasites with hu-RBC-SCID mice.
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pubmed:affiliation |
Department of Medical Zoology, Kobe 650-0017, School of Veterinary Medicine, Rakuno-gakuen University, Ebetsu 069-8501, Japan.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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