Source:http://linkedlifedata.com/resource/pubmed/id/11097267
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rdf:type | |
lifeskim:mentions |
umls-concept:C0005038,
umls-concept:C0012984,
umls-concept:C0030685,
umls-concept:C0205102,
umls-concept:C0205263,
umls-concept:C0391871,
umls-concept:C0553257,
umls-concept:C0596235,
umls-concept:C0680255,
umls-concept:C1283071,
umls-concept:C1550548,
umls-concept:C1555714,
umls-concept:C1705654,
umls-concept:C1963578
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pubmed:issue |
4
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pubmed:dateCreated |
2001-3-6
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pubmed:abstractText |
The effect of lindane (gamma-hexachlorocyclohexane), an organochlorine pesticide, on Ca2+ mobilization in Madin-Darby canine kidney cells was examined by fluorimetry using fura-2 as a Ca2+ indicator. Lindane (5-200 microM) increased [Ca2+]i concentration-dependently. The [Ca2+]i signal comprised an immediate initial rise followed by a persistent phase. Ca2+ removal inhibited the [Ca2+]i signal by reducing both the initial rise and the sustained phase. This implies lindane-triggered Ca2+ influx and Ca2+ release. In Ca2+ -free medium, 0.15 mM lindane increased [Ca2+]i after pretreatment with carbonylcyanide m-chlorophenylhydrazone (CCCP, 2 microM), a mitochondrial uncoupler, and two endoplasmic reticulum Ca2+ pump inhibitors, thapsigargin and cyclopiazonic acid. Conversely, pretreatment with lindane abolished CCCP- and thapsigargin-induced Ca2+ release. This suggests that 0.15 mM lindane released Ca2+ from the endoplasmic reticulum, mitochondria and other stores. La3+ (1 mM) partly inhibited 0.1 mM lindane-induced [Ca2+]i increase, confirming that lindane induced Ca2+ influx. Addition of 3 mM Ca2+ increased [Ca2+]i after pretreatment with 0.15 mM lindane for 750 sec. in Ca2+ -free medium, which indicates lindane-induced capacitative Ca2+ entry. Lindane (0.15 mM)-induced Ca2+ release was not reduced by inhibiting phospholipase C with 2 microM U73122, but was inhibited by 70% by the phospholipase A2 inhibitor aristolochic acid (40 microM).
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Calcium,
http://linkedlifedata.com/resource/pubmed/chemical/Carbonyl Cyanide m-Chlorophenyl...,
http://linkedlifedata.com/resource/pubmed/chemical/Enzyme Inhibitors,
http://linkedlifedata.com/resource/pubmed/chemical/Indoles,
http://linkedlifedata.com/resource/pubmed/chemical/Lindane,
http://linkedlifedata.com/resource/pubmed/chemical/Phospholipases,
http://linkedlifedata.com/resource/pubmed/chemical/Thapsigargin,
http://linkedlifedata.com/resource/pubmed/chemical/Uncoupling Agents,
http://linkedlifedata.com/resource/pubmed/chemical/cyclopiazonic acid
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pubmed:status |
MEDLINE
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pubmed:month |
Oct
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pubmed:issn |
0901-9928
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
87
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
149-55
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:11097267-Animals,
pubmed-meshheading:11097267-Calcium,
pubmed-meshheading:11097267-Carbonyl Cyanide m-Chlorophenyl Hydrazone,
pubmed-meshheading:11097267-Cells, Cultured,
pubmed-meshheading:11097267-Dogs,
pubmed-meshheading:11097267-Drug Interactions,
pubmed-meshheading:11097267-Endoplasmic Reticulum,
pubmed-meshheading:11097267-Enzyme Inhibitors,
pubmed-meshheading:11097267-Indoles,
pubmed-meshheading:11097267-Kidney,
pubmed-meshheading:11097267-Lindane,
pubmed-meshheading:11097267-Phospholipases,
pubmed-meshheading:11097267-Thapsigargin,
pubmed-meshheading:11097267-Uncoupling Agents
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pubmed:year |
2000
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pubmed:articleTitle |
Lindane (gamma-hexachlorocyclohexane) induces internal Ca2+ release and capacitative Ca2+ entry in Madin-Darby canine kidney cells.
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pubmed:affiliation |
Department of Neurology, Chang Gung Memorial Hospital-Kaohsiung, Kaohsiung Veterans General Hospital, Taiwan.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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