Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
2000-12-12
pubmed:abstractText
18S rDNA from 74 wine yeast strains was amplified by PCR using specific primers, and the products analyzed by temperature gradient gel electrophoresis (TGGE). TGGE is a useful method in screening the genotypes of the wine yeasts. Intraspecific differentiation was achieved on the basis of TGGE in some cases, whereas in others identical bands for strains classified as separate species were obtained. Heteroduplex analysis was capable of differentiating between similar bands produced by two different species, thereby enhancing the resolution of the TGGE, yielding valuable information in a short time without the need of sequencing or complicated equipment.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Dec
pubmed:issn
0378-1097
pubmed:author
pubmed:issnType
Print
pubmed:day
1
pubmed:volume
193
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
45-50
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
2000
pubmed:articleTitle
Characterization of wine yeasts by temperature gradient gel electrophoresis (TGGE).
pubmed:affiliation
Facultad de Ciencias Químicas, Universidad de Castilla-La Mancha, Ciudad Real, Spain.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't