Source:http://linkedlifedata.com/resource/pubmed/id/11093948
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
6
|
| pubmed:dateCreated |
2001-1-2
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| pubmed:abstractText |
We wished to determine whether exogenous glucagon-like peptide (GLP)-2 infusion stimulates intestinal growth in parenterally fed immature pigs. Piglets (106-108 days gestation) were given parenteral nutrient infusion (TPN), TPN + human GLP-2 (25 nmol. kg(-1). day(-1)), or sow's milk enterally (ENT) for 6 days. Intestinal protein synthesis was then measured in vivo after a bolus dose of [1-(13)C]phenylalanine, and degradation was calculated from the difference between protein accretion and synthesis. Crypt cell proliferation and apoptosis were measured in situ by 5-bromodeoxyuridine (BrdU) and terminal dUTP nick-end labeling (TUNEL), respectively. Intestinal protein and DNA accretion rates and villus heights were similar in GLP-2 and ENT pigs, and both were higher (P < 0.05) than in TPN pigs. GLP-2 decreased fractional protein degradation rate, whereas ENT increased fractional protein synthesis rate compared with TPN pigs. Percentage of TUNEL-positive cells in GLP-2 and ENT groups was 48 and 64% lower, respectively, than in TPN group (P < 0.05). However, ENT, but not GLP-2, increased percentage of BrdU-positive crypt cells above that in TPN piglets. We conclude that GLP-2 increases intestinal growth in premature, TPN-fed pigs by decreasing proteolysis and apoptosis, whereas enteral nutrition acts via increased protein synthesis and cell proliferation and decreased apoptosis.
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| pubmed:grant | |
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
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| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Dec
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| pubmed:issn |
0193-1857
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| pubmed:author | |
| pubmed:issnType |
Print
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| pubmed:volume |
279
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| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
G1249-56
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| pubmed:dateRevised |
2007-11-14
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| pubmed:meshHeading |
pubmed-meshheading:11093948-Animals,
pubmed-meshheading:11093948-Animals, Newborn,
pubmed-meshheading:11093948-Apoptosis,
pubmed-meshheading:11093948-Dietary Proteins,
pubmed-meshheading:11093948-Enteral Nutrition,
pubmed-meshheading:11093948-Female,
pubmed-meshheading:11093948-Glucagon-Like Peptide 2,
pubmed-meshheading:11093948-Glucagon-Like Peptides,
pubmed-meshheading:11093948-Humans,
pubmed-meshheading:11093948-In Situ Nick-End Labeling,
pubmed-meshheading:11093948-Intestine, Large,
pubmed-meshheading:11093948-Intestines,
pubmed-meshheading:11093948-Male,
pubmed-meshheading:11093948-Pancreas,
pubmed-meshheading:11093948-Parenteral Nutrition, Total,
pubmed-meshheading:11093948-Peptides,
pubmed-meshheading:11093948-Stomach,
pubmed-meshheading:11093948-Swine
|
| pubmed:year |
2000
|
| pubmed:articleTitle |
GLP-2 stimulates intestinal growth in premature TPN-fed pigs by suppressing proteolysis and apoptosis.
|
| pubmed:affiliation |
United States Department of Agriculture, Agricultural Research Service, Children's Nutrition Research Center, Department of Pediatrics, Baylor College of Medicine, Houston, Texas 77030, USA.
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| pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, U.S. Gov't, Non-P.H.S.,
Research Support, Non-U.S. Gov't
|