Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
6
pubmed:dateCreated
2000-12-18
pubmed:abstractText
The effect of trichostatin A (TSA), histone deacetylase inhibitor, on cell growth and the mechanism of growth modulation was examined in 8 gastric and 3 oral carcinoma cell lines which included 9-cis-retinoic acid resistant (MKN-7 and Ho-1-N-1) and IFN-beta resistant cell lines (MKN-7, -28 and -45). TSA inhibited growth in all cell lines examined. Apoptotic cell death was confirmed by apoptotic ladder formation and induction of a cleaved form (85 kDa) of poly (ADP-ribose) polymerase (PARP) induction. TSA enhanced the protein expression of p21(WAF1), CREB-binding protein, cyclinE, cyclin A, Bak and Bax, while it reduced the expression of E2F-1, E2F-4, HDAC1, p53 and hyperphosphorylated form of Rb. Furthermore, TSA induced morphological changes, such as elongation of cytoplasm and cell-to-cell detachment, in gastric and oral carcinoma cell lines. These results suggest that TSA may inhibit cell growth and induce apoptosis of gastric and oral carcinoma cells through modulation of the expression of cell cycle regulators and apoptosis-regulating proteins.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
http://linkedlifedata.com/resource/pubmed/chemical/BAK1 protein, human, http://linkedlifedata.com/resource/pubmed/chemical/BAX protein, human, http://linkedlifedata.com/resource/pubmed/chemical/Cell Cycle Proteins, http://linkedlifedata.com/resource/pubmed/chemical/Enzyme Inhibitors, http://linkedlifedata.com/resource/pubmed/chemical/Hydroxamic Acids, http://linkedlifedata.com/resource/pubmed/chemical/Membrane Proteins, http://linkedlifedata.com/resource/pubmed/chemical/PARP1 protein, human, http://linkedlifedata.com/resource/pubmed/chemical/Poly(ADP-ribose) Polymerases, http://linkedlifedata.com/resource/pubmed/chemical/Proteins, http://linkedlifedata.com/resource/pubmed/chemical/Proto-Oncogene Proteins, http://linkedlifedata.com/resource/pubmed/chemical/Proto-Oncogene Proteins c-bcl-2, http://linkedlifedata.com/resource/pubmed/chemical/Tumor Suppressor Protein p53, http://linkedlifedata.com/resource/pubmed/chemical/bcl-2 Homologous Antagonist-Killer..., http://linkedlifedata.com/resource/pubmed/chemical/bcl-2-Associated X Protein, http://linkedlifedata.com/resource/pubmed/chemical/trichostatin A
pubmed:status
MEDLINE
pubmed:month
Dec
pubmed:issn
0020-7136
pubmed:author
pubmed:copyrightInfo
Copyright 2000 Wiley-Liss, Inc.
pubmed:issnType
Print
pubmed:day
15
pubmed:volume
88
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
992-7
pubmed:dateRevised
2007-7-24
pubmed:meshHeading
pubmed-meshheading:11093826-Apoptosis, pubmed-meshheading:11093826-Cell Cycle Proteins, pubmed-meshheading:11093826-Cell Division, pubmed-meshheading:11093826-DNA Fragmentation, pubmed-meshheading:11093826-Drug Resistance, Neoplasm, pubmed-meshheading:11093826-Drug Screening Assays, Antitumor, pubmed-meshheading:11093826-Enzyme Inhibitors, pubmed-meshheading:11093826-Humans, pubmed-meshheading:11093826-Hydroxamic Acids, pubmed-meshheading:11093826-Membrane Proteins, pubmed-meshheading:11093826-Mouth Neoplasms, pubmed-meshheading:11093826-Poly(ADP-ribose) Polymerases, pubmed-meshheading:11093826-Proteins, pubmed-meshheading:11093826-Proto-Oncogene Proteins, pubmed-meshheading:11093826-Proto-Oncogene Proteins c-bcl-2, pubmed-meshheading:11093826-Stomach Neoplasms, pubmed-meshheading:11093826-Time Factors, pubmed-meshheading:11093826-Tumor Cells, Cultured, pubmed-meshheading:11093826-Tumor Suppressor Protein p53, pubmed-meshheading:11093826-bcl-2 Homologous Antagonist-Killer Protein, pubmed-meshheading:11093826-bcl-2-Associated X Protein
pubmed:year
2000
pubmed:articleTitle
Effect of trichostatin A on cell growth and expression of cell cycle- and apoptosis-related molecules in human gastric and oral carcinoma cell lines.
pubmed:affiliation
First Department of Pathology, Hiroshima University School of Medicine, Hiroshima, Japan.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't