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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
11
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pubmed:dateCreated |
2001-1-4
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pubmed:databankReference |
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pubmed:abstractText |
Interleukin (IL)-1-like protein 1 (IL-1L1) is a 155-amino acid protein that shares 27% identity with IL-1beta and 47% with IL-1 receptor antagonist (IL-1ra). A 2.7-kb IL-1L1 mRNA was cloned from human placenta and is detectable in the trophoblastic cell line JEG-3, in macrophages and in endotoxin-stimulated monocytes. Expression of IL-1L1 is much less abundant and less widespread than IL-1ra. We have determined the human and mouse IL-1L1 cDNA sequences and the complete sequence of the human gene, IL1L1. IL1L1 consists of four coding exons, has two alternative non-coding first exons, lies between IL1B and IL1RN, is orientated in the same direction as IL1RN and is separated from it by approximately 53 kb. The predicted IL-1L1 protein lacks both signal sequence and glycosylation signals. A 17-kDa protein was recovered by immunoprecipitation with IL-1L1-specific antibodies from JEG-3. IL-1L1 did not stimulate IL-6 production from primary human fibroblasts or human umbilical vein endothelial cells nor did it block the IL-1alpha or IL-1beta-dependent activation of IL-6 expression. We conclude, contrary to a recent suggestion made by others, that IL-1L1 is not a functional IL-1ra. IL-1L1 also had no detectable agonistic or antagonistic effect on IFN-gamma production in response to IL-18 in KG-1 cells.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/DNA, Complementary,
http://linkedlifedata.com/resource/pubmed/chemical/IL18R1 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/IL1RN protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Il18r1 protein, mouse,
http://linkedlifedata.com/resource/pubmed/chemical/Il1rn protein, mouse,
http://linkedlifedata.com/resource/pubmed/chemical/Interleukin 1 Receptor Antagonist...,
http://linkedlifedata.com/resource/pubmed/chemical/Interleukin-1,
http://linkedlifedata.com/resource/pubmed/chemical/Interleukin-18,
http://linkedlifedata.com/resource/pubmed/chemical/Interleukin-18 Receptor alpha...,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Interleukin,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Interleukin-1,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Interleukin-18,
http://linkedlifedata.com/resource/pubmed/chemical/Sialoglycoproteins
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pubmed:status |
MEDLINE
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pubmed:month |
Nov
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pubmed:issn |
0014-2980
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
30
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
3299-308
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:11093146-Amino Acid Sequence,
pubmed-meshheading:11093146-Animals,
pubmed-meshheading:11093146-DNA, Complementary,
pubmed-meshheading:11093146-Gene Expression Regulation,
pubmed-meshheading:11093146-Humans,
pubmed-meshheading:11093146-Interleukin 1 Receptor Antagonist Protein,
pubmed-meshheading:11093146-Interleukin-1,
pubmed-meshheading:11093146-Interleukin-18,
pubmed-meshheading:11093146-Interleukin-18 Receptor alpha Subunit,
pubmed-meshheading:11093146-Mice,
pubmed-meshheading:11093146-Molecular Sequence Data,
pubmed-meshheading:11093146-Multigene Family,
pubmed-meshheading:11093146-Organ Specificity,
pubmed-meshheading:11093146-Receptors, Interleukin,
pubmed-meshheading:11093146-Receptors, Interleukin-1,
pubmed-meshheading:11093146-Receptors, Interleukin-18,
pubmed-meshheading:11093146-Sequence Alignment,
pubmed-meshheading:11093146-Sialoglycoproteins,
pubmed-meshheading:11093146-Transcription, Genetic
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pubmed:year |
2000
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pubmed:articleTitle |
A tissue specific IL-1 receptor antagonist homolog from the IL-1 cluster lacks IL-1, IL-1ra, IL-18 and IL-18 antagonist activities.
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pubmed:affiliation |
Division of Molecular and Genetic Medicine, University of Sheffield, Sheffield, GB.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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