Source:http://linkedlifedata.com/resource/pubmed/id/11093122
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| rdf:type | |
| lifeskim:mentions |
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umls-concept:C2698600
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| pubmed:issue |
11
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| pubmed:dateCreated |
2001-1-4
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| pubmed:abstractText |
CD8(+) cytolytic T lymphocytes (CTL) play a fundamental role in the clearance of malaria parasites from the liver in mouse models. In humans, however, only low levels of parasite-specific CD8(+) T lymphocytes have been observed in individuals living in endemic areas. In the present study, we identified high levels of circulating CD8(+) T lymphocytes specific for a previously described HLA-A2-restricted CTL epitope of the circumsporozoite (CS) protein of Plasmodium falciparum in an adult living in Burkina Faso, as evidenced by IFN-gamma ELISPOT assay and MHC-tetramer technology. After cloning by limiting dilution culture, T cell recognition of natural CS variants of P. falciparum was studied. The results demonstrate that naturally occurring variations drastically affect residues critical for T cell recognition as only two out of nine sequences analyzed were efficiently recognized by the CTL clones. These clones were also used to analyze T cell recognition of the endogenously presented cognate antigen. We observed efficient antigen recognition of both HLA-A*0201-transfected murine antigen presenting cells and liver cells from HLA-A*0201/K(b)-transgenic mice upon infection with recombinant vaccinia virus encoding the CS protein (WR-CS). More importantly, we demonstrate for the first time efficient recognition of WR-CS-infected human liver cells.
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| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
IM
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| pubmed:chemical | |
| pubmed:status |
MEDLINE
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| pubmed:month |
Nov
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| pubmed:issn |
0014-2980
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| pubmed:author |
pubmed-author:BarbeyCC,
pubmed-author:BoneloAA,
pubmed-author:ChampagnePP,
pubmed-author:CorradinGG,
pubmed-author:EspositoFF,
pubmed-author:HerreraSS,
pubmed-author:LópezJ AJA,
pubmed-author:MenthaGG,
pubmed-author:NebiéII,
pubmed-author:OberholzerJJ,
pubmed-author:RomeroJ FJF,
pubmed-author:RomeroPP,
pubmed-author:TiercyJ MJM,
pubmed-author:TriponezFF,
pubmed-author:ValmoriDD
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| pubmed:issnType |
Print
|
| pubmed:volume |
30
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
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| pubmed:pagination |
3079-88
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| pubmed:dateRevised |
2006-11-15
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| pubmed:meshHeading |
pubmed-meshheading:11093122-Adult,
pubmed-meshheading:11093122-Animals,
pubmed-meshheading:11093122-Antigen Presentation,
pubmed-meshheading:11093122-Antigens, Protozoan,
pubmed-meshheading:11093122-CD8-Positive T-Lymphocytes,
pubmed-meshheading:11093122-Cell Differentiation,
pubmed-meshheading:11093122-Cytotoxicity, Immunologic,
pubmed-meshheading:11093122-Humans,
pubmed-meshheading:11093122-Liver,
pubmed-meshheading:11093122-Malaria,
pubmed-meshheading:11093122-Mice,
pubmed-meshheading:11093122-Plasmodium falciparum,
pubmed-meshheading:11093122-Polymorphism, Genetic,
pubmed-meshheading:11093122-Receptors, Antigen, T-Cell
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| pubmed:year |
2000
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| pubmed:articleTitle |
Generation and characterization of malaria-specific human CD8(+) lymphocyte clones: effect of natural polymorphism on T cell recognition and endogenous cognate antigen presentationby liver cells.
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| pubmed:affiliation |
Institute of Biochemistry, University of Lausanne, Epalinges, Switzerland.
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| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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