Source:http://linkedlifedata.com/resource/pubmed/id/11092735
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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
3-4
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pubmed:dateCreated |
2001-3-8
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pubmed:databankReference | |
pubmed:abstractText |
A 1200-bp cDNA encoding Xenopus laevis deoxyribonuclease I (X. laevis DNase I) was constructed from the total RNA of a X. laevis pancreas using a rapid amplification of cDNA ends method. When the cDNA was transiently transfected into COS-7 cells, the recombinant polypeptide exhibited similar enzymological properties to those of the native pancreatic DNase I. The recombinant enzyme was considerably more labile than most other vertebrate DNase I enzymes. The X. laevis DNase I polypeptide was larger than any other known vertebrate DNase I, containing a unique Cys-rich stretch of 68 or 70 amino acid residues at the carboxyl terminus, and it had less well conserved binding sites for the Ca2+, G-actin and DNA, and two DNase I signature motifs. These alterations might account for its heat instability.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical | |
pubmed:status |
MEDLINE
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pubmed:issn |
1042-5179
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
11
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
247-55
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:11092735-Amino Acid Sequence,
pubmed-meshheading:11092735-Animals,
pubmed-meshheading:11092735-Base Sequence,
pubmed-meshheading:11092735-COS Cells,
pubmed-meshheading:11092735-Cercopithecus aethiops,
pubmed-meshheading:11092735-Cloning, Molecular,
pubmed-meshheading:11092735-DNA, Complementary,
pubmed-meshheading:11092735-Deoxyribonuclease I,
pubmed-meshheading:11092735-Humans,
pubmed-meshheading:11092735-Kinetics,
pubmed-meshheading:11092735-Molecular Sequence Data,
pubmed-meshheading:11092735-Pancreas,
pubmed-meshheading:11092735-Phylogeny,
pubmed-meshheading:11092735-Recombinant Proteins,
pubmed-meshheading:11092735-Transfection,
pubmed-meshheading:11092735-Vertebrates,
pubmed-meshheading:11092735-Xenopus laevis
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pubmed:year |
2000
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pubmed:articleTitle |
Molecular cloning of cDNA encoding Xenopus laevis deoxyribonuclease I.
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pubmed:affiliation |
Department of Legal Medicine, Gunma University School of Medicine, Maebashi, Japan.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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