Linked Life Data

11091903

Source:http://linkedlifedata.com/resource/pubmed/id/11091903

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
5-6
pubmed:dateCreated
2001-2-20
pubmed:abstractText
Sex determination in the mammalian embryo begins with the activation of a gene on the Y chromosome which triggers a cascade of events that lead to male development. The mechanism by which this gene, designated SRY in humans and Sry in mice (sex determining region of the Y chromosome), is activated remains unknown. Likewise, the downstream target genes for Sry remain unidentified at present. C57BL mice carrying a Y chromosome from Mus musculus musculus or molossinus develop normally as males. In contrast, C57BL/6 mice with the Y chromosome from M. m. domesticus often show sex reversal, i.e., develop as XY females. It has been documented that C57BL mice with the Y chromosome from Poschiavinus (YPOS), a domesticus subtype, always develop as females or hermaphrodites. This suggests that a C57BL gene either up- or downstream of Sry is ineffective in interacting with Sry, which then compromises the processes that lead to normal male sex development. Nonetheless, by selective breeding, we have been able to generate a sex reversal-resistant C57BL/6-congenic strain of mice in which the XYPOS individuals consistently develop as normal males with bilateral testes. Because the resistance to sex reversal was transferred from strain 129S1/Sv (nonalbino) by simple selection over 13 backcross generations, it is inferred that a single autosomal gene or chromosomal region confers resistance to the sex reversal that would otherwise result. XYPOS normal males generated in these crosses were compared to XYPOS abnormal individuals and to C57BL/6 controls for sexual phenotype, gonadal weight, serum testosterone, and major urinary protein (MUP) level. A clear correlation was found among phenotypic sex, MUP level, and testis weight in the males and in the incompletely masculinized XYPOS mice. The fully masculinized males of the congenic strain resemble C57BL/6 males in the tested parameters. DNA analysis confirmed that these males, in fact, carry the YPOS Sry gene.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jun
pubmed:issn
0006-2928
pubmed:author
pubmed:issnType
Print
pubmed:volume
38
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
119-37
pubmed:dateRevised
2010-11-18
pubmed:meshHeading
pubmed-meshheading:11091903-Alpha-Globulins, pubmed-meshheading:11091903-Animals, pubmed-meshheading:11091903-DNA-Binding Proteins, pubmed-meshheading:11091903-Disorders of Sex Development, pubmed-meshheading:11091903-Female, pubmed-meshheading:11091903-Male, pubmed-meshheading:11091903-Mice, pubmed-meshheading:11091903-Mice, Congenic, pubmed-meshheading:11091903-Mice, Inbred C57BL, pubmed-meshheading:11091903-Nuclear Proteins, pubmed-meshheading:11091903-Organ Size, pubmed-meshheading:11091903-Ovary, pubmed-meshheading:11091903-Phenotype, pubmed-meshheading:11091903-Polymorphism, Restriction Fragment Length, pubmed-meshheading:11091903-Proteins, pubmed-meshheading:11091903-Sex Determination Processes, pubmed-meshheading:11091903-Sex-Determining Region Y Protein, pubmed-meshheading:11091903-Testis, pubmed-meshheading:11091903-Testosterone, pubmed-meshheading:11091903-Transcription Factors, pubmed-meshheading:11091903-X Chromosome, pubmed-meshheading:11091903-Y Chromosome
pubmed:year
2000
pubmed:articleTitle
A single genetic determinant that prevents sex reversal in C57BL-YPOS congenic mice.
pubmed:affiliation
Department of Biochemistry and Molecular Biology, Medical College of Georgia, Augusta 30912, USA.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't