Linked Life Data

11091002

Source:http://linkedlifedata.com/resource/pubmed/id/11091002

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
2000-12-26
pubmed:abstractText
The extensive role of the microsomal mixed-function oxidase (MFO) system in the oxidation of endo-and xenobiotics, in the detoxication, in the generation of reactive free radicals and in the decomposition of the end products of lipid peroxides is well documented in the literature. Steatotic liver is a very frequent damage with different etiology. Drug metabolising reactions are suppressed in fatty liver, in which pathologically increased production of reactive oxygen intermediates may lead to the peroxidation of microsomal membrane lipids and to the change of membrane bound enzyme activities because of overwhelmed protective mechanisms. The subnormal activity of the MFO system may diminish the non specific resistance of the organism. Therefore we have studied the effects of natural flavonoids and polyphenolic compounds on the mixed-function oxidases. Antioxidant, O(2)(-&z.rad;) and &z. rad;OH scavenger properties of Sempervivum tectorum extract (STF1) were proved by EPR spectroscopic and chemiluminometric techniques. Potential bioactive constituents were determined by chromatography (HPLC, TLC) and spectrometric (UV, UV-VIS) methods. In the present study we reflect on the membrane stabilising, antioxidant and lipid metabolism modifying effects of this extract. It was established that activities of NAD(P)H reductase and content of cytochrome P450 were normalised in liver microsomes of hyperlipidemic rats, if the animals were treated with STF1 (2 g/bwkg for 9 days in drinking water parallel with fat-rich diet feeding). Fatty acid composition, examined by HRGLC analysis, was changed beneficially. NADPH induced lipid peroxidation was also decreased in microsomes in in vivo and in vitro experiments. At the same time the STF1 had no significant influence on MFO system in normolipidemic animals and on cytochrome b5 concentration of microsome fractions of hyperlipidemic rats.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Dec
pubmed:issn
0378-8741
pubmed:author
pubmed:issnType
Print
pubmed:volume
73
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
479-85
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
2000
pubmed:articleTitle
Membrane stabilising effects of natural polyphenols and flavonoids from Sempervivum tectorum on hepatic microsomal mixed-function oxidase system in hyperlipidemic rats.
pubmed:affiliation
Second Department of Medicine, Semmelweis University, H-1088, Szentkirályi St. 46., Budapest, Hungary. blaz@bel2.sote.hu
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't