Linked Life Data

11090542

Source:http://linkedlifedata.com/resource/pubmed/id/11090542

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
11
pubmed:dateCreated
2000-12-1
pubmed:abstractText
During heart development, 2 fast-conducting regions of working myocardium balloon out from the slow-conducting primary myocardium of the tubular heart. Three regions of primary myocardium persist: the outflow tract, atrioventricular canal, and inflow tract, which are contiguous throughout the inner curvature of the heart. The contribution of the inflow tract to the definitive atrial chambers has remained enigmatic largely because of the lack of molecular markers that permit unambiguous identification of this myocardial domain. We now report that the genes encoding atrial natriuretic factor, myosin light chain (MLC) 3F, MLC2V, and Pitx-2, and transgenic mouse lines expressing nlacZ under the control of regulatory sequences of the mouse MLC1F/3F gene, display regionalized patterns of expression in the atrial component of the developing mouse heart. These data distinguish 4 broad transcriptional domains in the atrial myocardium: (1) the atrioventricular canal that will form the smooth-walled lower atrial rim proximal to the ventricles; (2) the atrial appendages; (3) the caval vein myocardium (systemic inlet); and (4) the mediastinal myocardium (pulmonary inlet), including the atrial septa. The pattern of expression of Pitx-2 reveals that each of these transcriptional domains has a distinct left and right component. This study reveals for the first time differential gene expression in the systemic and pulmonary inlets, which is not shared by the contiguous atrial appendages and provides evidence for multiple molecular compartments within the atrial chambers. Furthermore, this work will allow the contribution of each of these myocardial components to be studied in congenitally malformed hearts, such as those with abnormal venous return.
pubmed:commentsCorrections
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Nov
pubmed:issn
1524-4571
pubmed:author
pubmed:issnType
Electronic
pubmed:day
24
pubmed:volume
87
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
984-91
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed-meshheading:11090542-Aging, pubmed-meshheading:11090542-Animals, pubmed-meshheading:11090542-Antigens, Differentiation, pubmed-meshheading:11090542-Atrial Natriuretic Factor, pubmed-meshheading:11090542-Gene Expression Regulation, Developmental, pubmed-meshheading:11090542-Genes, Reporter, pubmed-meshheading:11090542-Heart Atria, pubmed-meshheading:11090542-Homeodomain Proteins, pubmed-meshheading:11090542-Lac Operon, pubmed-meshheading:11090542-Mice, pubmed-meshheading:11090542-Mice, Inbred C57BL, pubmed-meshheading:11090542-Mice, Transgenic, pubmed-meshheading:11090542-Myocardium, pubmed-meshheading:11090542-Myosin Light Chains, pubmed-meshheading:11090542-Nuclear Proteins, pubmed-meshheading:11090542-Organ Specificity, pubmed-meshheading:11090542-Paired Box Transcription Factors, pubmed-meshheading:11090542-Rats, pubmed-meshheading:11090542-Rats, Wistar, pubmed-meshheading:11090542-Transcription, Genetic, pubmed-meshheading:11090542-Transcription Factors, pubmed-meshheading:11090542-Transgenes
pubmed:year
2000
pubmed:articleTitle
Multiple transcriptional domains, with distinct left and right components, in the atrial chambers of the developing heart.
pubmed:affiliation
Experimental and Molecular Cardiology Group, Academic Medical Center, University of Amsterdam, Amsterdam, the Netherlands. dfranco@ujaen.es
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't