Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
5496
pubmed:dateCreated
2000-11-30
pubmed:abstractText
We generated a mutant of the red fluorescent protein drFP583. The mutant (E5) changes its fluorescence from green to red over time. The rate of color conversion is independent of protein concentration and therefore can be used to trace time-dependent expression. We used in vivo labeling with E5 to measure expression from the heat shock-dependent promoter in Caenorhabditis elegans and from the Otx-2 promoter in developing Xenopus embryos. Thus, E5 is a "fluorescent timer" that can be used to monitor both activation and down-regulation of target promoters on the whole-organism scale.
pubmed:grant
pubmed:commentsCorrections
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Nov
pubmed:issn
0036-8075
pubmed:author
pubmed:issnType
Print
pubmed:day
24
pubmed:volume
290
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1585-8
pubmed:dateRevised
2008-11-21
pubmed:meshHeading
pubmed-meshheading:11090358-Animals, pubmed-meshheading:11090358-Brain, pubmed-meshheading:11090358-Caenorhabditis elegans, pubmed-meshheading:11090358-Cell Line, pubmed-meshheading:11090358-Color, pubmed-meshheading:11090358-Fluorescence, pubmed-meshheading:11090358-Gene Expression Profiling, pubmed-meshheading:11090358-Gene Expression Regulation, pubmed-meshheading:11090358-Gene Expression Regulation, Developmental, pubmed-meshheading:11090358-Heat-Shock Proteins, pubmed-meshheading:11090358-Homeodomain Proteins, pubmed-meshheading:11090358-Humans, pubmed-meshheading:11090358-Luminescent Proteins, pubmed-meshheading:11090358-Mutation, pubmed-meshheading:11090358-Nerve Tissue Proteins, pubmed-meshheading:11090358-Otx Transcription Factors, pubmed-meshheading:11090358-Promoter Regions, Genetic, pubmed-meshheading:11090358-Temperature, pubmed-meshheading:11090358-Time Factors, pubmed-meshheading:11090358-Trans-Activators, pubmed-meshheading:11090358-Xenopus laevis
pubmed:year
2000
pubmed:articleTitle
"Fluorescent timer": protein that changes color with time.
pubmed:affiliation
School of Medicine, Stanford University, Stanford, CA 94305, USA. Alexey.Terskikh@Stanford.edu
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't