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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
5496
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pubmed:dateCreated |
2000-11-30
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pubmed:abstractText |
Evidence for a new signaling mechanism consisting of ligand-independent lateral propagation of receptor activation in the plasma membrane is presented. We visualized the phosphorylation of green fluorescent protein (GFP)-tagged ErbB1 (ErbB1-GFP) receptors in cells focally stimulated with epidermal growth factor (EGF) covalently attached to beads. This was achieved by quantitative imaging of protein reaction states in cells by fluorescence resonance energy transfer (FRET) with global analysis of fluorescence lifetime imaging microscopy (FLIM) data. The rapid and extensive propagation of receptor phosphorylation over the entire cell after focal stimulation demonstrates a signaling wave at the plasma membrane resulting in full activation of all receptors.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Arsenicals,
http://linkedlifedata.com/resource/pubmed/chemical/Carbocyanines,
http://linkedlifedata.com/resource/pubmed/chemical/Enzyme Inhibitors,
http://linkedlifedata.com/resource/pubmed/chemical/Epidermal Growth Factor,
http://linkedlifedata.com/resource/pubmed/chemical/Fluorescent Dyes,
http://linkedlifedata.com/resource/pubmed/chemical/Green Fluorescent Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Immunoglobulin Fab Fragments,
http://linkedlifedata.com/resource/pubmed/chemical/Ligands,
http://linkedlifedata.com/resource/pubmed/chemical/Luminescent Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Phosphotyrosine,
http://linkedlifedata.com/resource/pubmed/chemical/Protein Tyrosine Phosphatases,
http://linkedlifedata.com/resource/pubmed/chemical/Receptor, Epidermal Growth Factor,
http://linkedlifedata.com/resource/pubmed/chemical/cyanine dye 3,
http://linkedlifedata.com/resource/pubmed/chemical/oxophenylarsine
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pubmed:status |
MEDLINE
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pubmed:month |
Nov
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pubmed:issn |
0036-8075
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
24
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pubmed:volume |
290
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1567-70
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pubmed:dateRevised |
2009-11-19
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pubmed:meshHeading |
pubmed-meshheading:11090353-Arsenicals,
pubmed-meshheading:11090353-Carbocyanines,
pubmed-meshheading:11090353-Cell Membrane,
pubmed-meshheading:11090353-Diffusion,
pubmed-meshheading:11090353-Dimerization,
pubmed-meshheading:11090353-Endocytosis,
pubmed-meshheading:11090353-Energy Transfer,
pubmed-meshheading:11090353-Enzyme Inhibitors,
pubmed-meshheading:11090353-Epidermal Growth Factor,
pubmed-meshheading:11090353-Fluorescence,
pubmed-meshheading:11090353-Fluorescent Dyes,
pubmed-meshheading:11090353-Green Fluorescent Proteins,
pubmed-meshheading:11090353-Humans,
pubmed-meshheading:11090353-Immunoglobulin Fab Fragments,
pubmed-meshheading:11090353-Ligands,
pubmed-meshheading:11090353-Luminescent Proteins,
pubmed-meshheading:11090353-Microscopy, Confocal,
pubmed-meshheading:11090353-Microscopy, Fluorescence,
pubmed-meshheading:11090353-Microspheres,
pubmed-meshheading:11090353-Phosphorylation,
pubmed-meshheading:11090353-Phosphotyrosine,
pubmed-meshheading:11090353-Protein Tyrosine Phosphatases,
pubmed-meshheading:11090353-Receptor, Epidermal Growth Factor,
pubmed-meshheading:11090353-Signal Transduction,
pubmed-meshheading:11090353-Tumor Cells, Cultured
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pubmed:year |
2000
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pubmed:articleTitle |
Quantitative imaging of lateral ErbB1 receptor signal propagation in the plasma membrane.
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pubmed:affiliation |
Cell Biology and Cell Biophysics Program, European Molecular Biology Laboratory, Meyerhofstrasse 1, 69117 Heidelberg, Germany.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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