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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
24
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pubmed:dateCreated |
2001-1-11
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pubmed:abstractText |
The retroviral primary transcription product is a multifunctional RNA that is utilized as pre-mRNA, mRNA, and genomic RNA. The relationship between human immunodeficiency virus type 1 (HIV-1) unspliced transcripts used as mRNA for viral protein synthesis and as virion precursor RNA (vpRNA) for encapsidation remains an important question. We developed a biochemical assay to evaluate the hypothesis that prior utilization as mRNA template for protein synthesis is necessary to generate vpRNA. HIV-1-infected T cells were treated with translation inhibitors under conditions that maintain virus production. Immunoprecipitation of newly synthesized HIV-1 Gag protein revealed that de novo translation is not necessary to sustain assembly, release, or processing of Gag structural protein. Both newly synthesized protein and steady-state Gag are competent for assembly, and the extracellular accumulation of Gag is proportional to the intracellular abundance of Gag. As early as 2 h after transcription, newly synthesized RNA is detectable in cell-free virions and encapsidation is sustained upon inhibition of host cell translation. Results of both [(3)H]uridine incorporation assays and HIV-1-specific RNase protection assays (RPAs) indicate that translation inhibition reduces the absolute amounts of both cytoplasmic and virion-associated RNA. Evaluation of encapsidation efficiency by RPA revealed that the cytoplasmic availability of vpRNA is increased, indicating that HIV-1 unspliced mRNA can be rerouted to function as vpRNA. Our data contrast with results from the HIV-2 and murine leukemia virus systems and indicate that HIV-1 unspliced RNA constitutes a single functional pool that can function interchangeably as mRNA and as vpRNA.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/11090150-10074152,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11090150-10233946,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11090150-10933721,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11090150-1172324,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11090150-1354856,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11090150-2353454,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11090150-4134468,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11090150-4859409,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11090150-5541758,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11090150-57617,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11090150-5960401,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11090150-6172599,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11090150-6311951,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11090150-7958838,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11090150-8551634,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11090150-8602510,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11090150-8627772,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11090150-8794299,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11090150-9094696,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11090150-9151848,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11090150-9420292,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11090150-976261
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Dec
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pubmed:issn |
0022-538X
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
74
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
11531-7
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
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pubmed:year |
2000
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pubmed:articleTitle |
Translation is not required To generate virion precursor RNA in human immunodeficiency virus type 1-infected T cells.
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pubmed:affiliation |
Center for Retrovirus Research, The Ohio State University, Columbus, Ohio 43210-1093, USA.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.
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