|
rdf:type |
|
|
lifeskim:mentions |
|
|
pubmed:issue |
7
|
|
pubmed:dateCreated |
2000-12-20
|
|
pubmed:abstractText |
1. We investigated the effect of oestradiol on basolateral potassium channels in human colonic epithelium. 2. Ion transport was quantified using short circuit current (I:(sc)) measurements of samples mounted in Ussing chambers. Serosal K transport was studied using nystatin permeabilization of the apical membrane. Intracellular pH changes were quantified using spectroflouresence techniques. 3. Experiments were performed with either 10 nM or 1 microM Ca(2+) in the apical bathing solution. With 10 nM Ca(2+) in the apical bathing solution addition of oestradiol (1 nM) to the basolateral bath produced a rapid increase in current (delta I(K)=11.2+/-1.2 microA.cm(-2), n=6). This response was prevented by treatment of the serosal membrane with tolbutamide (1 microM). With 1 microM Ca(2+) in the apical bathing solution addition of oestradiol produced a rapid fall in current (delta I(K)=-12.8+/-1.4 microA.cm(-2)), this response was prevented by treatment of the basolateral membrane with tetra-pentyl-ammonium (TPeA). These responses were rapid and occurred independently of protein synthesis. 4. Inhibition of basolateral Na(+)/H(+) exchange with either amiloride or a low sodium bathing solution prevented this response. These responses were prevented by inhibition of protein kinase C (PKC) with bis-indolyl-maleimide. 5. Oestradiol (1 nM) produced a rapid intracellular alkanization (mean increase=0.11 pH units; n=6; P<0.01). 6. These results suggest that oestradiol rapidly modulates serosal K transport in human colon. These effects depend upon intact Na(+)/H(+) exchange and protein kinase C. We propose a non-classical, possibly membrane linked, mechanism for oestradiol action in human colonic epithelium.
|
|
pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/11090109-10323673,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11090109-1372288,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11090109-181580,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11090109-2138421,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11090109-2535787,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11090109-2553716,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11090109-4745226,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11090109-633356,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11090109-6370117,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11090109-7720682,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11090109-7931572,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11090109-834244,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11090109-8382884,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11090109-8759093,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11090109-8770001,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11090109-9000458,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11090109-9011603,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11090109-915470,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11090109-9316461,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11090109-9526711
|
|
pubmed:language |
eng
|
|
pubmed:journal |
|
|
pubmed:citationSubset |
IM
|
|
pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Calcium,
http://linkedlifedata.com/resource/pubmed/chemical/Cycloheximide,
http://linkedlifedata.com/resource/pubmed/chemical/Estradiol,
http://linkedlifedata.com/resource/pubmed/chemical/Estrogen Antagonists,
http://linkedlifedata.com/resource/pubmed/chemical/Potassium,
http://linkedlifedata.com/resource/pubmed/chemical/Potassium Channels,
http://linkedlifedata.com/resource/pubmed/chemical/Protein Synthesis Inhibitors,
http://linkedlifedata.com/resource/pubmed/chemical/Quaternary Ammonium Compounds,
http://linkedlifedata.com/resource/pubmed/chemical/Sodium-Hydrogen Antiporter,
http://linkedlifedata.com/resource/pubmed/chemical/Tamoxifen,
http://linkedlifedata.com/resource/pubmed/chemical/Tolbutamide,
http://linkedlifedata.com/resource/pubmed/chemical/tetrapentylammonium
|
|
pubmed:status |
MEDLINE
|
|
pubmed:month |
Dec
|
|
pubmed:issn |
0007-1188
|
|
pubmed:author |
|
|
pubmed:issnType |
Print
|
|
pubmed:volume |
131
|
|
pubmed:owner |
NLM
|
|
pubmed:authorsComplete |
Y
|
|
pubmed:pagination |
1373-8
|
|
pubmed:dateRevised |
2009-11-18
|
|
pubmed:meshHeading |
pubmed-meshheading:11090109-Calcium,
pubmed-meshheading:11090109-Colon,
pubmed-meshheading:11090109-Cycloheximide,
pubmed-meshheading:11090109-Dose-Response Relationship, Drug,
pubmed-meshheading:11090109-Estradiol,
pubmed-meshheading:11090109-Estrogen Antagonists,
pubmed-meshheading:11090109-Humans,
pubmed-meshheading:11090109-Hydrogen-Ion Concentration,
pubmed-meshheading:11090109-Ion Transport,
pubmed-meshheading:11090109-Membrane Potentials,
pubmed-meshheading:11090109-Potassium,
pubmed-meshheading:11090109-Potassium Channels,
pubmed-meshheading:11090109-Protein Synthesis Inhibitors,
pubmed-meshheading:11090109-Quaternary Ammonium Compounds,
pubmed-meshheading:11090109-Serous Membrane,
pubmed-meshheading:11090109-Sodium-Hydrogen Antiporter,
pubmed-meshheading:11090109-Tamoxifen,
pubmed-meshheading:11090109-Tolbutamide
|
|
pubmed:year |
2000
|
|
pubmed:articleTitle |
Rapid activation of basolateral potassium transport in human colon by oestradiol.
|
|
pubmed:affiliation |
Department of Clinical Neurophysiology, Box 124, Addenbrookes Hospital, Cambridge, CB2 2QQ.
|
|
pubmed:publicationType |
Journal Article,
In Vitro
|
