Source:http://linkedlifedata.com/resource/pubmed/id/11090057
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
12
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| pubmed:dateCreated |
2000-12-11
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| pubmed:abstractText |
Tie-2 receptor tyrosine kinase expressed in endothelial and hematopoietic cells is believed to play a role in both angiogenesis and hematopoiesis during development of the mouse embryo. This article addressed whether Tie-2 is expressed on fetal liver hematopoietic stem cells (HSCs) at day 14 of gestation. With the use of anti-Tie-2 monoclonal antibody, its expression was detected in approximately 7% of an HSC population of Kit-positive, Sca-1-positive, lineage-negative or -low, and AA4.1-positive (KSLA) cells. These Tie-2-positive KSLA (T(+) KSLA) cells represent 0.01% to 0.02% of fetal liver cells. In vitro colony and in vivo competitive repopulation assays were performed for T(+) KSLA cells and Tie-2-negative KSLA (T(-) KSLA) cells. In the presence of stem cell factor, interleukin-3, and erythropoietin, 80% of T(+) KSLA cells formed colonies in vitro, compared with 40% of T(-) KSLA cells. Long-term multilineage repopulating cells were detected in T(+) KSLA cells, but not in T(-) KSLA cells. An in vivo limiting dilution analysis revealed that at least 1 of 8 T(+) KSLA cells were such repopulating cells. The successful secondary transplantation initiated with a limited number of T(+) KSLA cells suggests that these cells have self-renewal potential. In addition, engraftment of T(+) KSLA cells in conditioned newborn mice indicates that these HSCs can be adapted equally by the adult and newborn hematopoietic environments. The data suggest that T(+) KSLA cells represent HSCs in the murine fetal liver. (Blood. 2000;96:3757-3762)
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| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
AIM
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| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antibodies, Monoclonal,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, Ly,
http://linkedlifedata.com/resource/pubmed/chemical/Ly6a protein, mouse,
http://linkedlifedata.com/resource/pubmed/chemical/Membrane Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Proto-Oncogene Proteins c-kit,
http://linkedlifedata.com/resource/pubmed/chemical/Receptor, TIE-2,
http://linkedlifedata.com/resource/pubmed/chemical/Receptor Protein-Tyrosine Kinases
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| pubmed:status |
MEDLINE
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| pubmed:month |
Dec
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| pubmed:issn |
0006-4971
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| pubmed:author | |
| pubmed:issnType |
Print
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| pubmed:day |
1
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| pubmed:volume |
96
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| pubmed:owner |
NLM
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| pubmed:authorsComplete |
Y
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| pubmed:pagination |
3757-62
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| pubmed:dateRevised |
2009-11-19
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| pubmed:meshHeading |
pubmed-meshheading:11090057-Animals,
pubmed-meshheading:11090057-Antibodies, Monoclonal,
pubmed-meshheading:11090057-Antigens, Ly,
pubmed-meshheading:11090057-Bone Marrow Cells,
pubmed-meshheading:11090057-Cell Lineage,
pubmed-meshheading:11090057-Female,
pubmed-meshheading:11090057-Fetus,
pubmed-meshheading:11090057-Hematopoiesis,
pubmed-meshheading:11090057-Hematopoietic Stem Cell Transplantation,
pubmed-meshheading:11090057-Hematopoietic Stem Cells,
pubmed-meshheading:11090057-Liver,
pubmed-meshheading:11090057-Male,
pubmed-meshheading:11090057-Membrane Proteins,
pubmed-meshheading:11090057-Mice,
pubmed-meshheading:11090057-Mice, Inbred C57BL,
pubmed-meshheading:11090057-Mice, Mutant Strains,
pubmed-meshheading:11090057-Phenotype,
pubmed-meshheading:11090057-Proto-Oncogene Proteins c-kit,
pubmed-meshheading:11090057-Receptor, TIE-2,
pubmed-meshheading:11090057-Receptor Protein-Tyrosine Kinases,
pubmed-meshheading:11090057-Stem Cells,
pubmed-meshheading:11090057-Transplantation Chimera
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| pubmed:year |
2000
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| pubmed:articleTitle |
Hematopoietic stem cells express Tie-2 receptor in the murine fetal liver.
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| pubmed:affiliation |
Department of Immunology, Institute of Basic Medical Sciences, University of Tsukuba, Japan.
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| pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, Non-U.S. Gov't
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