Linked Life Data

11089553

Source:http://linkedlifedata.com/resource/pubmed/id/11089553

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
11
pubmed:dateCreated
2000-11-29
pubmed:abstractText
Glucagon-like peptide-2 (GLP-2) is secreted by enteroendocrine cells in the small and large intestines and exerts intestinotropic effects in the gastrointestinal mucosal epithelium of the adult rodent. The actions of GLP-2 are mediated by the GLP-2 receptor, a new member of the G protein-coupled receptor superfamily. To ascertain whether the GLP-2/GLP-2 receptor axis is expressed and functional in the developing intestine, we have studied the synthesis of GLP-2 and the expression of the GLP-2 receptor (GLP-2R) in the fetal and neonatal rat gut. GLP-2 immunoreactivity (GLP-2-IR) was detected in the fetal rat intestine, and fetal rat intestinal cell cultures secreted correctly processed GLP-2(1-33) into the medium. High levels of GLP-2(1-33) were also detected in the circulation of 13-day-old neonatal rats (P < 0.001 vs. adult). Analysis of GLP-2 receptor expression by RT-PCR demonstrated GLP-2R messenger RNA transcripts in fetal intestine and in neonatal stomach, jejunum, ileum, and colon. The levels of GLP-2R messenger RNA transcripts were comparatively higher in the fetal and neonatal intestine (P < 0.05-001 vs. adult) and declined to adult levels by postnatal day 21. Subcutaneous administration of a degradation-resistant GLP-2 analog, h[Gly2]-GLP-2 once daily for 10 days increased stomach (0.009 +/- 0.0003 vs. 0.007 +/- 0.002 g/g body mass, h[Gly2]-GLP-2-treated vs. controls; P < 0.05) and small bowel weight (0.043 +/- 0.0037 vs. 0.031 +/- 0.0030 g/g body mass; P < 0.05). h[Gly2]-GLP-2 also increased both small (2.4 +/- 0.05 vs. 1.8 +/- 0.17 cm/g body mass; P < 0.05) and large bowel length (0.32 +/- 0.01 vs. 0.25 +/- 0.02 cm/g body mass, h[Gly2]-GLP-2-treated vs. saline-treated controls, respectively; P < 0.05) in neonatal rats. These findings demonstrate that both components of the GLP-2/GLP-2 receptor axis are expressed in the fetal and neonatal intestine. The ontogenic regulation and functional integrity of this axis raises the possibility that GLP-2 may play a role in the development and/or maturation of the developing rat intestine.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
AIM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Nov
pubmed:issn
0013-7227
pubmed:author
pubmed:issnType
Print
pubmed:volume
141
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
4194-201
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed-meshheading:11089553-Animals, pubmed-meshheading:11089553-Animals, Newborn, pubmed-meshheading:11089553-Colon, pubmed-meshheading:11089553-Gene Expression, pubmed-meshheading:11089553-Glucagon, pubmed-meshheading:11089553-Glucagon-Like Peptide 2, pubmed-meshheading:11089553-Glucagon-Like Peptides, pubmed-meshheading:11089553-Intestine, Small, pubmed-meshheading:11089553-Intestines, pubmed-meshheading:11089553-Organ Size, pubmed-meshheading:11089553-Peptides, pubmed-meshheading:11089553-Proglucagon, pubmed-meshheading:11089553-Protein Precursors, pubmed-meshheading:11089553-RNA, Messenger, pubmed-meshheading:11089553-Rats, pubmed-meshheading:11089553-Rats, Wistar, pubmed-meshheading:11089553-Receptors, Glucagon, pubmed-meshheading:11089553-Reverse Transcriptase Polymerase Chain Reaction, pubmed-meshheading:11089553-Stomach
pubmed:year
2000
pubmed:articleTitle
Ontogeny of the glucagon-like peptide-2 receptor axis in the developing rat intestine.
pubmed:affiliation
Department of Physiology, Toronto General Hospital, Banting and Best Diabetes Center, University of Toronto, Ontario, Canada.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't