Source:http://linkedlifedata.com/resource/pubmed/id/11087231
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
6
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| pubmed:dateCreated |
2000-12-15
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| pubmed:abstractText |
We investigated the effects of estrogen on global myocardial ischemia-reperfusion injury in rats that were ovariectomized (Ovx), sham-operated, or ovariectomized and then given 17beta-estradiol (E(2)beta) supplementation (Ovx+E(2)beta). Hearts were excised, cannulated, perfused with and then immersed in chilled (4 degrees C) cardioplegia solution for 30 min, and then retrogradely perfused with warm (37 degrees C), oxygenated Krebs-Henseleit bicarbonate buffer for 120 min. The coronary flow rate, first derivative of left ventricular pressure, and nitrite production were all significantly lower in Ovx than in sham-operated or Ovx+E(2)beta hearts. However, coronary flow rates or nitrate production were not consistently different throughout the entire reperfusion period. Ca(2+) accumulated more in Ovx rat hearts than in sham-operated or Ovx+E(2)beta hearts, and mitochondrial respiratory function was lower in Ovx hearts than in hearts from the other two groups. Marked interstitial edema and contraction bands were seen in hematoxylin-eosin-stained sections of Ovx rat hearts but not in hearts from either of the other groups. Hematoxylin-basic fuchsin-picric acid-stained sections revealed fewer viable myocytes in hearts from the Ovx group than from the sham or Ovx+E(2)beta group. Transmission electron microscopy demonstrated more severely damaged mitochondria and ultrastructural damage to myocytes in Ovx rat hearts. Our results indicate that estrogen plays a cardioprotective role in global myocardial ischemia-reperfusion injury in female rats.
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| pubmed:grant | |
| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
IM
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| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Calcium,
http://linkedlifedata.com/resource/pubmed/chemical/Coloring Agents,
http://linkedlifedata.com/resource/pubmed/chemical/Estradiol,
http://linkedlifedata.com/resource/pubmed/chemical/Nitric Oxide,
http://linkedlifedata.com/resource/pubmed/chemical/Nitrites,
http://linkedlifedata.com/resource/pubmed/chemical/Tetrazolium Salts,
http://linkedlifedata.com/resource/pubmed/chemical/Thiazoles,
http://linkedlifedata.com/resource/pubmed/chemical/thiazolyl blue
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| pubmed:status |
MEDLINE
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| pubmed:month |
Dec
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| pubmed:issn |
0363-6135
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| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
279
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
H2766-75
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| pubmed:dateRevised |
2007-11-14
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| pubmed:meshHeading |
pubmed-meshheading:11087231-Animals,
pubmed-meshheading:11087231-Calcium,
pubmed-meshheading:11087231-Coloring Agents,
pubmed-meshheading:11087231-Coronary Circulation,
pubmed-meshheading:11087231-Estradiol,
pubmed-meshheading:11087231-Female,
pubmed-meshheading:11087231-Microscopy, Electron,
pubmed-meshheading:11087231-Mitochondria,
pubmed-meshheading:11087231-Myocardial Reperfusion Injury,
pubmed-meshheading:11087231-Myocardium,
pubmed-meshheading:11087231-Nitric Oxide,
pubmed-meshheading:11087231-Nitrites,
pubmed-meshheading:11087231-Ovariectomy,
pubmed-meshheading:11087231-Rats,
pubmed-meshheading:11087231-Rats, Sprague-Dawley,
pubmed-meshheading:11087231-Tetrazolium Salts,
pubmed-meshheading:11087231-Thiazoles,
pubmed-meshheading:11087231-Ventricular Function, Left,
pubmed-meshheading:11087231-Ventricular Pressure
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| pubmed:year |
2000
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| pubmed:articleTitle |
Effect of estrogen on global myocardial ischemia-reperfusion injury in female rats.
|
| pubmed:affiliation |
Department of Veterinary Biosciences, University of Illinois, Urbana-Champaign, Illinois 61802, USA.
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| pubmed:publicationType |
Journal Article,
In Vitro,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
|