Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
11
pubmed:dateCreated
2000-12-7
pubmed:databankReference
pubmed:abstractText
Multiple cis-acting elements including the intronic enhancer and the 3'alpha enhancer (3'alphaE) regulate expression of the Ig heavy chain genes during B cell development. A 3'alphaE is composed of DNase I-hypersensitive sites, hs1,2, hs3a,b, and hs4, found 3' of the murine Calpha gene as well as 3' of both human Calpha genes, Calpha1 and Calpha2. Rabbits have 13 Calpha genes, and we tested whether a 3'alphaE is associated with each of these genes. To identify 3'alphaE regions we developed a rabbit hs1,2 probe and used this to search for enhancer homologues of human hs1,2 in a genomic fosmid library. We identified a single hs1,2 fragment 8-kb downstream of Calpha13, the presumed 3'-most Calpha gene. We also identified and partially sequenced a new Calpha gene, Calpha14, located 6 kb upstream of Calpha13. Genomic Southern blot analysis confirmed that the rabbit genome contains only one hs1,2 enhancer region. We tested the enhancer activity of the hs1,2 with the SV40, V(H), and Ialpha promoters using the luciferase reporter gene in transient transfection assays and found that it significantly enhanced the activity of SV40 and V(H) promoters and slightly enhanced an Ialpha promoter. We conclude that the rabbit has a single hs1,2 enhancer that resides at the 3' end of the IgH gene cluster and may constitute one of the cis-elements regulating the expression of IgH genes.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
AIM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Dec
pubmed:issn
0022-1767
pubmed:author
pubmed:issnType
Print
pubmed:day
1
pubmed:volume
165
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
6400-5
pubmed:dateRevised
2008-11-21
pubmed:meshHeading
pubmed-meshheading:11086078-3' Untranslated Regions, pubmed-meshheading:11086078-Amino Acid Motifs, pubmed-meshheading:11086078-Animals, pubmed-meshheading:11086078-Base Sequence, pubmed-meshheading:11086078-Blotting, Southern, pubmed-meshheading:11086078-Cell Line, pubmed-meshheading:11086078-Cloning, Molecular, pubmed-meshheading:11086078-DNA Probes, pubmed-meshheading:11086078-DNA-Binding Proteins, pubmed-meshheading:11086078-Enhancer Elements, Genetic, pubmed-meshheading:11086078-Genetic Vectors, pubmed-meshheading:11086078-Humans, pubmed-meshheading:11086078-Immunoglobulin Constant Regions, pubmed-meshheading:11086078-Immunoglobulin Heavy Chains, pubmed-meshheading:11086078-Mice, pubmed-meshheading:11086078-Molecular Sequence Data, pubmed-meshheading:11086078-Multigene Family, pubmed-meshheading:11086078-Physical Chromosome Mapping, pubmed-meshheading:11086078-Promoter Regions, Genetic, pubmed-meshheading:11086078-Rabbits, pubmed-meshheading:11086078-Restriction Mapping, pubmed-meshheading:11086078-Sequence Homology, Nucleic Acid, pubmed-meshheading:11086078-Tumor Cells, Cultured
pubmed:year
2000
pubmed:articleTitle
A single 3' alpha hs1,2 enhancer in the rabbit IgH locus.
pubmed:affiliation
Department of Microbiology and Immunology, Loyola University Chicago, Maywood, IL 60153, USA.
pubmed:publicationType
Journal Article, Comparative Study, Research Support, U.S. Gov't, P.H.S.