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pubmed-article:11083856pubmed:abstractTextWe have observed previously that attachment of Toxoplasma gondii to synchronized host cells is considerably increased at the mid-S phase (4 h postrelease). Synchronized CHO host cells at the mid-S phase were fractionated by molecular weight, and the antigens were used to produce a panel of polyclonal mouse antisera. The polyclonal antisera raised against fraction 4 with molecular mass ranging approximately from 18 to 40 kDa significantly reduced attachment to mid-S-phase host cells. Immunofluorescence assays demonstrated strong reactivity to mid-S-phase host cells and identified a number of potential receptors on Western blots. These data indicate that there is a specific host membrane receptor for parasite attachment that is upregulated during the mid-S phase of the host cell cycle.lld:pubmed
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pubmed-article:11083856pubmed:authorpubmed-author:KasperL HLHlld:pubmed
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pubmed-article:11083856pubmed:dateRevised2009-11-18lld:pubmed
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pubmed-article:11083856pubmed:articleTitleAttachment of Toxoplasma gondii to a specific membrane fraction of CHO cells.lld:pubmed
pubmed-article:11083856pubmed:affiliationDepartment of Medicine and Microbiology, Dartmouth Medical School, Lebanon, New Hampshire 03756, USA.lld:pubmed
pubmed-article:11083856pubmed:publicationTypeJournal Articlelld:pubmed
pubmed-article:11083856pubmed:publicationTypeResearch Support, U.S. Gov't, P.H.S.lld:pubmed