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rdf:type |
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lifeskim:mentions |
umls-concept:C0040557,
umls-concept:C0085080,
umls-concept:C0185023,
umls-concept:C0205369,
umls-concept:C0237497,
umls-concept:C1166621,
umls-concept:C1264633,
umls-concept:C1314972,
umls-concept:C1947904,
umls-concept:C1999228,
umls-concept:C2825781
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pubmed:issue |
12
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pubmed:dateCreated |
2000-12-13
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pubmed:abstractText |
We have observed previously that attachment of Toxoplasma gondii to synchronized host cells is considerably increased at the mid-S phase (4 h postrelease). Synchronized CHO host cells at the mid-S phase were fractionated by molecular weight, and the antigens were used to produce a panel of polyclonal mouse antisera. The polyclonal antisera raised against fraction 4 with molecular mass ranging approximately from 18 to 40 kDa significantly reduced attachment to mid-S-phase host cells. Immunofluorescence assays demonstrated strong reactivity to mid-S-phase host cells and identified a number of potential receptors on Western blots. These data indicate that there is a specific host membrane receptor for parasite attachment that is upregulated during the mid-S phase of the host cell cycle.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/11083856-10027960,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11083856-10225919,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11083856-10455025,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11083856-1399003,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11083856-15275469,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11083856-1534073,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11083856-1641648,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11083856-1944419,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11083856-4876978,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11083856-6018562,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11083856-6833761,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11083856-8050521,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11083856-8926075,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11083856-9208224,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11083856-9573113
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Dec
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pubmed:issn |
0019-9567
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
68
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
7198-201
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:11083856-Animals,
pubmed-meshheading:11083856-CHO Cells,
pubmed-meshheading:11083856-Cell Membrane,
pubmed-meshheading:11083856-Cricetinae,
pubmed-meshheading:11083856-Fluorescent Antibody Technique, Indirect,
pubmed-meshheading:11083856-Membrane Proteins,
pubmed-meshheading:11083856-Mice,
pubmed-meshheading:11083856-Mice, Inbred BALB C,
pubmed-meshheading:11083856-Molecular Weight,
pubmed-meshheading:11083856-Rabbits,
pubmed-meshheading:11083856-S Phase,
pubmed-meshheading:11083856-Toxoplasma
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pubmed:year |
2000
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pubmed:articleTitle |
Attachment of Toxoplasma gondii to a specific membrane fraction of CHO cells.
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pubmed:affiliation |
Department of Medicine and Microbiology, Dartmouth Medical School, Lebanon, New Hampshire 03756, USA.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.
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